受激布里渊散射流式细胞术
Stimulated Brillouin scattering flow cytometry.
作者信息
Rosvold Jake R, Zanini Giulia, Handler Chenchen, Frank Eric, Li Jiarui, Vitolo Michele I, Martin Stuart S, Scarcelli Giuliano
机构信息
Fischell Department of Bioengineering, University of Maryland, 8278 Paint Branch Drive, College Park, MD 20742, USA.
Department of Mechanical Engineering, A. James Clark School of Engineering, University of Maryland, College Park, MD 20742, USA.
出版信息
Biomed Opt Express. 2024 Sep 25;15(10):6024-6035. doi: 10.1364/BOE.537602. eCollection 2024 Oct 1.
We present the use of stimulated Brillouin scattering spectroscopy to achieve rapid measurements of cell biomechanics in a flow cytometer setup. Specifically, our stimulated Brillouin scattering flow cytometry can acquire at a rate of 200 Hz, with a spectral acquisition time of 5 ms, which marks a 10x improvement compared to previous demonstrations of spontaneous Brillouin scattering flow cytometry. We experimentally validate our stimulated Brillouin scattering flow cytometer by measuring cell populations of normal breast epithelial cells and metastatic breast epithelial cancer cells.
我们展示了在流式细胞仪设置中使用受激布里渊散射光谱来实现细胞生物力学的快速测量。具体而言，我们的受激布里渊散射流式细胞仪能够以200 Hz的速率采集数据，光谱采集时间为5毫秒，与之前自发布里渊散射流式细胞仪的演示相比，这标志着有10倍的改进。我们通过测量正常乳腺上皮细胞和转移性乳腺上皮癌细胞的细胞群体，对我们的受激布里渊散射流式细胞仪进行了实验验证。
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