Biomedical Engineering Department, Ben-Gurion University of the Negev, Be'er-Sheva, Israel.
Agilent Research Laboratories, Petach Tikva, Israel.
Nat Methods. 2020 Sep;17(9):913-916. doi: 10.1038/s41592-020-0882-0. Epub 2020 Aug 3.
Label-free, non-contact imaging with mechanical contrast and optical sectioning is a substantial challenge in microscopy. Spontaneous Brillouin scattering microscopy meets this challenge, but encounters a trade-off between acquisition speed and the specificity for biomechanical constituents with overlapping Brillouin bands. Stimulated Brillouin scattering microscopy overcomes this trade-off and enables the cross-sectional imaging of live Caenorhabditis elegans at the organ and subcellular levels, with both elasticity and viscosity contrasts at high specificity and with practical recording times.
无标记、非接触的力学对比和光学切片成像在显微镜技术中是一项重大挑战。自发布里渊散射显微镜技术满足了这一挑战，但在采集速度和对具有重叠布里渊频带的生物力学成分的特异性之间存在折衷。受激布里渊散射显微镜技术克服了这一折衷，实现了活体秀丽隐杆线虫在器官和亚细胞水平的横截面成像，具有高特异性的弹性和粘性对比，并且具有实际的记录时间。
