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用于小分子检测的羧甲基壳聚糖功能化载玻片的斜入射反射率差分技术开发。

Development of a carboxymethyl chitosan functionalized slide for small molecule detection using oblique-incidence reflectivity difference technology.

作者信息

Xu Mengjing, Shi Boyang, Li Haofeng, Mai Xiaohan, Mi Lan, Ma Jiong, Zhu Xiangdong, Wang Guowei, Fei Yiyan

机构信息

Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Information Science and Technology, Fudan University, Shanghai 200433, China.

Quzhou Fudan Institute, 108 Minjiang Avenue, Kecheng District, Quzhou, Zhejiang Province, China.

出版信息

Biomed Opt Express. 2024 Sep 19;15(10):5947-5959. doi: 10.1364/BOE.534563. eCollection 2024 Oct 1.

DOI:10.1364/BOE.534563
PMID:39421793
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11482164/
Abstract

Label-free optical biosensors have become powerful tools in the study of biomolecular interactions without the need for labels. High throughput and low detection limit are desirable for rapid and accurate biomolecule detection. The oblique-incidence reflectivity difference (OI-RD) technique is capable of detecting thousands of biomolecular interactions in a high-throughput mode, specifically for biomolecules larger than 1000 Da. In order to enhance the detection capability of OI-RD for small molecules (typically < 500 Da), we have developed a three-dimensional biochip that utilized carboxymethyl chitosan (CMCS) functionalized slides. By investigating various factors such as sonication time, protein immobilization time, CMCS molecular weight, and glutaraldehyde (GA) functionalization time, we have achieved a detection limit of 6.8 pM for avidin (68 kDa). Furthermore, accurate detection of D-biotin with a molecular weight of 244 Da has also been achieved. This paper presents an effective solution for achieving both high throughput and low detection limits using the OI-RD technique in the field of biomolecular interaction detection.

摘要

无标记光学生物传感器已成为研究生物分子相互作用的强大工具，无需使用标记物。高吞吐量和低检测限对于快速准确地检测生物分子是理想的。斜入射反射率差（OI-RD）技术能够以高通量模式检测数千种生物分子相互作用，特别适用于大于1000 Da的生物分子。为了提高OI-RD对小分子（通常<500 Da）的检测能力，我们开发了一种利用羧甲基壳聚糖（CMCS）功能化载玻片的三维生物芯片。通过研究诸如超声处理时间、蛋白质固定时间、CMCS分子量和戊二醛（GA）功能化时间等各种因素，我们实现了对抗生物素蛋白（68 kDa）6.8 pM的检测限。此外，还实现了对分子量为244 Da的D-生物素的准确检测。本文提出了一种在生物分子相互作用检测领域使用OI-RD技术实现高吞吐量和低检测限的有效解决方案。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f7/11482164/1bad9179690f/boe-15-10-5947-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f7/11482164/ca7a9a7213ce/boe-15-10-5947-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f7/11482164/a330547f94be/boe-15-10-5947-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f7/11482164/a481c0bb4baa/boe-15-10-5947-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f7/11482164/b780a7befff7/boe-15-10-5947-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f7/11482164/187724e0a077/boe-15-10-5947-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f7/11482164/50ae755f79ab/boe-15-10-5947-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f7/11482164/e4ee59c2231a/boe-15-10-5947-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f7/11482164/1bad9179690f/boe-15-10-5947-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f7/11482164/ca7a9a7213ce/boe-15-10-5947-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f7/11482164/a330547f94be/boe-15-10-5947-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f7/11482164/a481c0bb4baa/boe-15-10-5947-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f7/11482164/b780a7befff7/boe-15-10-5947-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f7/11482164/187724e0a077/boe-15-10-5947-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f7/11482164/50ae755f79ab/boe-15-10-5947-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f7/11482164/e4ee59c2231a/boe-15-10-5947-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1f7/11482164/1bad9179690f/boe-15-10-5947-g008.jpg

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