Zhang Hang, Xu Mengjing, Li Haofeng, Mai Xiaohan, Sun Jiawei, Mi Lan, Ma Jiong, Zhu Xiangdong, Fei Yiyan
Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-Precision Optical Manufacturing, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), School of Information Science and Technology, Fudan University, Shanghai, 200433, China.
Department of Science and Technology, Shanghai Deyu Intelligent Technology Co., Ltd., Shanghai, 201413, China.
Biomed Opt Express. 2023 Apr 28;14(5):2386-2399. doi: 10.1364/BOE.487563. eCollection 2023 May 1.
The oblique-incidence reflectivity difference (OI-RD) microscope is a label-free detection system for microarrays that has many successful applications in high throughput drug screening. The increase and optimization of the detection speed of the OI-RD microscope will enable it to be a potential ultra-high throughput screening tool. This work presents a series of optimization methods that can significantly reduce the time to scan an OI-RD image. The wait time for the lock-in amplifier was decreased by the proper selection of the time constant and development of a new electronic amplifier. In addition, the time for the software to acquire data and for translation stage movement was also minimized. As a result, the detection speed of the OI-RD microscope is 10 times faster than before, making the OI-RD microscope suitable for ultra-high throughput screening applications.
斜入射反射率差(OI-RD)显微镜是一种用于微阵列的无标记检测系统,在高通量药物筛选中有许多成功应用。OI-RD显微镜检测速度的提高和优化将使其成为一种潜在的超高通量筛选工具。这项工作提出了一系列优化方法,可显著减少扫描OI-RD图像的时间。通过适当选择时间常数和开发新型电子放大器,减少了锁定放大器的等待时间。此外,软件获取数据和转台移动的时间也被最小化。结果,OI-RD显微镜的检测速度比以前快了10倍,使OI-RD显微镜适用于超高通量筛选应用。