You Chunmei, Zhang Lulu, Song Jiajia, Zhen Congai, Ji Xiangyun, Zhang Lei, Gao Xiwu
Eco-Environmental Protection Institution, Shanghai Academy of Agricultural Science, Shanghai, China.
Department of Entomology, China Agricultural University, Beijing, China.
Pest Manag Sci. 2025 Feb;81(2):831-838. doi: 10.1002/ps.8487. Epub 2024 Oct 18.
The house fly is a public health pest due to its ability to transmit various pathogens worldwide. The suppression of house flies is frequently controlled by organophosphate and carbamate insecticides, but recurrent selection has led to the mutated target. To understand the spread and evolution of Mdace mutations in house flies, we sampled flies in eight different China provinces, sequenced, and then estimated the frequencies of mutations.
The inhibition of AChE towards propoxur varied geographically and was lowest in the Heilongjiang field population, and highest in Jiangsu population. The overall frequencies of the different Mdace mutations (V260L, G342A, G342V, F407Y) varied considerably between populations. Strong positive correlations were found between acetylcholinesterase (AChE) insensitivity and the frequencies of G342V allele, 342A/V genotype and combination (260V/L-342A/V-407Y/Y) for the field populations. Eighteen combinations of Mdace at three sites (V260L, G342A/V, F407Y) were observed, with the majority of flies being categorized into the combination (260V/L-342A/V-407Y/Y), ranging in frequency from 24% (in field populations in Hubei and Hainan) to 96% (Jiangsu).
Based on comparison with historical data, the mutations responsible for the extremely high resistance to the level of insecticide resistance spread widely, propoxur should be limited to use for house fly control in China. © 2024 Society of Chemical Industry.
家蝇能够在全球传播各种病原体，是一种公共卫生害虫。家蝇的防治通常依靠有机磷和氨基甲酸酯类杀虫剂，但反复的选择导致了靶标的突变。为了解家蝇中Mdace突变的传播和进化情况，我们在中国八个不同省份采集了家蝇样本，进行测序，然后估计突变频率。
乙酰胆碱酯酶（AChE）对残杀威的抑制作用在地理上存在差异，在黑龙江田间种群中最低，在江苏种群中最高。不同Mdace突变（V260L、G342A、G342V、F407Y）的总体频率在不同种群之间差异很大。对于田间种群，发现乙酰胆碱酯酶（AChE）不敏感性与G342V等位基因频率、342A/V基因型以及组合（260V/L - 342A/V - 407Y/Y）之间存在强正相关。观察到三个位点（V260L、G342A/V、F407Y）的Mdace有18种组合，大多数家蝇被归类为组合（260V/L - 342A/V - 407Y/Y），频率范围从24%（湖北和海南的田间种群）到96%（江苏）。
与历史数据相比，导致对杀虫剂产生极高抗性水平的突变广泛传播，在中国应限制使用残杀威来防治家蝇。© 2024化学工业协会。
