School of Agricultural, Environmental and Veterinary Sciences, Faculty of Science and Health, Charles Sturt University, Wagga Wagga, New South Wales, Australia.
Biosecurity Research Program and Training Centre, Gulbali Institute, Charles Sturt University, Wagga Wagga, New South Wales, Australia.
Vet Med Sci. 2024 Nov;10(6):e70083. doi: 10.1002/vms3.70083.
Birds are known to harbour many pathogens, including circovirus, herpesviruses, adenoviruses and Chlamydia psittaci. Some of these pose zoonotic risks, while others, such as beak and feather disease virus (BFDV), have a significant impact on the conservation of endangered bird species.
This study was aimed to determine the faecal virome of a group of apparently healthy Monk parakeet using high-throughput sequencing.
Fresh faecal samples were collected from four Monk parakeets at a pet shop in Melbourne, Australia. Virus enrichment and nucleic acid extraction were performed on the faecal samples, followed by high-throughput sequencing at the Australian Genome Research Facility (AGRF).
Utilising an established pipeline for high-throughput sequencing data analysis, this study revealed the presence of three viruses of the families Circoviridae, Parvoviridae and Adenoviridae. Subsequent sequence comparison and phylogenetic analyses further confirmed that the detected viruses belong to the genera Chaphamaparvovirus (unassigned species), Circovirus (species Circovirus parrot) and Siadenovirus (species Siadenovirus viridis).
Despite non-pathogenicity, the existence of multiple viruses within a bird species underscores the risk of these viruses spreading into the pet trade. Detection and a better understanding of avian viruses are crucial for the establishment of appropriate management and biosecurity measures in the domestic and international bird trade, which ultimately supports the conservation of vulnerable bird species.
众所周知，鸟类携带许多病原体，包括圆环病毒、疱疹病毒、腺病毒和鹦鹉热衣原体。其中一些具有人畜共患风险，而另一些则如喙羽病病毒（BFDV），对濒危鸟类物种的保护具有重大影响。
本研究旨在使用高通量测序来确定一组表现健康的和尚鹦鹉的粪便病毒组。
从澳大利亚墨尔本一家宠物店的四只和尚鹦鹉中采集新鲜粪便样本。对粪便样本进行病毒富集和核酸提取，然后在澳大利亚基因组研究设施（AGRF）进行高通量测序。
利用高通量测序数据分析的既定管道，本研究揭示了三种病毒科 Circoviridae、Parvoviridae 和 Adenoviridae 的存在。随后的序列比较和系统发育分析进一步证实，检测到的病毒属于 Chaphamaparvovirus 属（未指定种）、Circovirus 属（Circovirus parrot 种）和 Siadenovirus 属（Siadenovirus viridis 种）。
尽管这些病毒无致病性，但在一个鸟类物种中存在多种病毒，突显了这些病毒传播到宠物贸易中的风险。检测和更好地了解禽病毒对于在国内外鸟类贸易中建立适当的管理和生物安全措施至关重要，这最终支持了脆弱鸟类物种的保护。
