Dietzgen R G
Arch Virol. 1986;87(1-2):73-86. doi: 10.1007/BF01310544.
Monoclonal antibodies to TMV vulgare produced in hybridoma cultures as well as in ascitic fluid were characterized according to their reactivity with the virion and/or the coat protein monomer thus revealing specificity for epitopes, cryptotopes or neotopes. Different patterns of crossreactivity of these monoclonal antibodies with TMV strains dahlemense and Holmes' Ribgrass occurred. Some monoclonal antibodies showed stronger reactivity with these strains than with the immunizing strain. The monoclonal antibodies were TMV-specific as they did not react with ArMV and PLRV and proteins of healthy tobacco plants. The monoclonal antibodies were of the IgG2a or IgM isotype. The specific activity (Ext405nm/hour/100 micrograms MCA) with the immunizing virus and its coat protein monomers was determined as characteristic property of each monoclonal antibody. A monoclonal antibody specific for the C-terminal epitope of TMV coat protein was selected by means of the corresponding chemically synthesized tetrapeptide. With this monoclonal antibody infectivity of TMV was neutralized.
对在杂交瘤培养物以及腹水中产生的针对普通烟草花叶病毒（TMV）的单克隆抗体，根据它们与病毒粒子和/或衣壳蛋白单体的反应性进行了表征，从而揭示了对表位、隐蔽表位或新表位的特异性。这些单克隆抗体与达勒姆烟草花叶病毒（TMV strains dahlemense）和霍姆斯狗尾草花叶病毒（Holmes' Ribgrass）存在不同的交叉反应模式。一些单克隆抗体与这些病毒株的反应性比与免疫原性病毒株更强。这些单克隆抗体具有TMV特异性，因为它们不与苜蓿花叶病毒（ArMV）、马铃薯卷叶病毒（PLRV）以及健康烟草植株的蛋白质发生反应。这些单克隆抗体属于IgG2a或IgM同种型。测定了与免疫原性病毒及其衣壳蛋白单体的比活性（Ext405nm/小时/100微克单克隆抗体），作为每种单克隆抗体的特征性质。通过相应的化学合成四肽筛选出了一种对TMV衣壳蛋白C末端表位具有特异性的单克隆抗体。用这种单克隆抗体可中和TMV的感染性。
