用于体内细胞治疗的低密度脂蛋白油红O染色方案。
Protocol for oil red O staining of low-density lipoproteins for in vivo cell treatment.
作者信息
Bini Simone, Covino Stella, Minicocci Ilenia, D'Erasmo Laura, Tramontano Daniele, Di Costanzo Alessia, Arca Marcello, Pecce Valeria
机构信息
Sapienza University of Rome, Department of Translational and Precision Medicine, 00185 Rome, Italy.
Sapienza University of Rome, Department of Translational and Precision Medicine, 00185 Rome, Italy.
出版信息
STAR Protoc. 2024 Dec 20;5(4):103376. doi: 10.1016/j.xpro.2024.103376. Epub 2024 Oct 18.
Low-density lipoproteins (LDLs) are the most abundant circulating lipoproteins and the most critical factor in the development of atherosclerosis. This protocol allows the staining of LDLs with oil red O to monitor particle uptake in bright-field microscopy. Here, we describe how to stain isolated LDLs using oil red O and how to use them to monitor LDL uptake in time-lapse experiments or fixed cells.
低密度脂蛋白（LDLs）是循环中含量最丰富的脂蛋白，也是动脉粥样硬化发展过程中最关键的因素。本实验方案允许使用油红O对LDLs进行染色，以便在明场显微镜下监测颗粒摄取情况。在这里，我们描述了如何使用油红O对分离出的LDLs进行染色，以及如何在延时实验或固定细胞中使用它们来监测LDL摄取情况。
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