通过CRISPR/Cas9基因组编辑技术建立CIB1基因敲除的人多能干细胞系。
Establishment of a CIB1 knockout human pluripotent stem cell line via CRISPR/Cas9 genome editing technology.
作者信息
Gong Tingyu, Liu Dandan, Wang Xiaochen, Zhou Danni, Tang Ling, Wang Hao, Su Jun, Liang Ping
机构信息
Shulan International Medical College, Zhejiang Shuren University, Hangzhou, 310015, China.
Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China; Institute of Translational Medicine, Zhejiang University, Hangzhou 310029, China.
出版信息
Stem Cell Res. 2024 Dec;81:103580. doi: 10.1016/j.scr.2024.103580. Epub 2024 Oct 15.
Calcium- and integrin-binding protein 1 (CIB1) has a diverse role in many different cell types and processes, including calcium signaling, migration, adhesion, proliferation, and survival. It is associated with cancer, cardiovascular disease and male infertility. Here, CRISPR/Cas9 genome-editing technology was employed to establish a CIB1 knockout human embryonic stem cell line, which exhibited normal pluripotency and karyotype.
钙整合素结合蛋白1（CIB1）在许多不同细胞类型和过程中具有多种作用，包括钙信号传导、迁移、黏附、增殖和存活。它与癌症、心血管疾病和男性不育症有关。在此，采用CRISPR/Cas9基因组编辑技术建立了一个CIB1基因敲除的人类胚胎干细胞系，该细胞系表现出正常的多能性和核型。
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