Saleem Amina, Wei Mingyu, Abbas Muhammad Khawar, Zhang Siyao, Fan Jiaqi, Xian Yang, Jiang Hongfeng
Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing 100029, China.
Department of Cardiology, Peking University Third Hospital, 49 Huayuan North Road, Haidian District, Beijing 100191, China.
Stem Cell Res. 2025 Mar;83:103642. doi: 10.1016/j.scr.2024.103642. Epub 2024 Dec 28.
Pyruvate Dehydrogenase Kinase1 (PDK1) belongs to the family of kinases, regulates diverse metabolic processes. PDK1 is a susceptibility locus for heart failure via thinning of ventricle walls, and enlarged atria and ventricles. We successfully developed a PDK1 knockout (PDK1/) human embryonic stem cell (hESC) line using an episomal vector-based CRISPR/Cas9 system explore the role of PDK in human heart development. This PDK1-KO hESC line-maintained stem cell-like morphology, pluripotency, and normal karyotype and can differentiate into all three germ layers in vivo. This cell line will be a valuable tool for future research on the role of PDK1 in heart development.
丙酮酸脱氢酶激酶1（PDK1）属于激酶家族，可调节多种代谢过程。PDK1是通过心室壁变薄以及心房和心室增大导致心力衰竭的一个易感基因座。我们使用基于附加型载体的CRISPR/Cas9系统成功开发了一种PDK1基因敲除（PDK1 -/-）人胚胎干细胞（hESC）系，以探究PDK在人类心脏发育中的作用。该PDK1基因敲除的hESC系保持了干细胞样形态、多能性和正常核型，并且在体内可分化为所有三个胚层。该细胞系将成为未来研究PDK1在心脏发育中作用的宝贵工具。
