Kumar Ram Surath, Ankola Anil V, Nagamoti Mahantesh B, Sankeshwari Roopali M, Sutar Kishori P, Jigan Shushant I, Pai Khot Atrey J
Department of Public Health Dentistry, KLE Vishwanath Katti Institute of Dental Sciences, KLE Academy of Higher Education and Research, Belagavi, 590010, India.
Department of Public Health Dentistry, KLE Vishwanath Katti Institute of Dental Sciences, KLE Academy of Higher Education and Research, Belagavi, 590010, India.
J Ayurveda Integr Med. 2024 Sep-Oct;15(5):101046. doi: 10.1016/j.jaim.2024.101046. Epub 2024 Oct 19.
Chlorhexidine (CHX) is considered as a gold standard for its antibacterial efficacy and substantivity in chemical plaque control. However, some adverse effects are associated with its prolonged use. Herbal medicines like Achyranthes aspera and Trachyspermum ammi have been used in many clinical conditions, and they appear to be a valuable substitute next to CHX in the management of periodontal diseases.
This in vitro study was designed to assess and compare the antibacterial potential and cytotoxic effects of novel polyherbal mouthwash containing A. aspera and T. ammi with 0.2% CHX mouthwash against Porphyromonas gingivalis, Tannerella forsythia, and Aggregatibacter actinomycetemcomitans.
Ethanolic extracts of A. aspera and T. ammi were prepared by the Soxhlet apparatus method and were subjected to preliminary phytochemical screening. The individual plant extracts and the plant extract mixture (PEM) of A. aspera and T. ammi in the ratio of 1:1, 2:1, 1:2 (w/v) were assessed for minimum inhibitory concentration (resazurin microtitre assay) and minimum bactericidal concentration (spread plating method) against selected periodontal pathogens in comparison to CHX. The polyherbal mouthwash was assessed for zone of inhibition (well diffusion method) and cytotoxicity (MTT assay) on adult human gingival fibroblasts. All the experiments were performed in triplicate.
The antibacterial activity was evident in the PEMs, and polyherbal mouthwash against tested periodontal pathogens and was comparable to CHX. The cytotoxicity assay findings confirmed that polyherbal mouthwash exhibited 82.1% of surviving cells which proved good biocompatibility.
A. aspera and T. ammi based mouthwash possess comparable antibacterial activity against periodontal pathogens when compared to CHX.
洗必泰（CHX）因其在化学菌斑控制中的抗菌效果和持续性而被视为金标准。然而，长期使用会产生一些不良反应。牛膝和孜然等草药已被用于多种临床病症，在牙周疾病的管理中，它们似乎是仅次于CHX的有价值替代品。
本体外研究旨在评估并比较含有牛膝和孜然的新型多草药漱口水与0.2%CHX漱口水对牙龈卟啉单胞菌、福赛坦纳菌和伴放线聚集杆菌的抗菌潜力及细胞毒性作用。
采用索氏提取器法制备牛膝和孜然的乙醇提取物，并进行初步植物化学筛选。将单味植物提取物以及牛膝和孜然按1:1、2:1、1:2（w/v）比例混合的植物提取物混合物（PEM）与CHX比较，评估其对所选牙周病原体的最低抑菌浓度（刃天青微量滴定法）和最低杀菌浓度（平板涂布法）。采用打孔扩散法评估多草药漱口水对成人牙龈成纤维细胞的抑菌圈，并采用MTT法评估其细胞毒性。所有实验均重复三次。
PEMs及多草药漱口水对受试牙周病原体具有明显的抗菌活性，且与CHX相当。细胞毒性试验结果证实，多草药漱口水有82.1%的存活细胞，证明其具有良好的生物相容性。
与CHX相比，基于牛膝和孜然的漱口水对牙周病原体具有相当的抗菌活性。
