Pathan Multazim Muradkhan, Bhat Kishore Gajanan, Joshi Vinayak Mahableshwar
Department of Molecular Biology and Immunology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India.
Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Central Research Laboratory, Belgaum, Karnataka, India.
J Indian Soc Periodontol. 2017 Jul-Aug;21(4):270-275. doi: 10.4103/jisp.jisp_382_16.
Several herbal mouthwash and herbal extracts have been tested and in search of a suitable adjunct to mechanical therapy for long-term use. In this study, we aimed to look at the antimicrobial effect of the herbal mouthwash and chlorhexidine (CHX) mouthwash on select organisms in test and an model.
The antimicrobial effects were determined against standard strains of bacteria that are involved in different stages of periodontal diseases. The tests included determination of minimum inhibitory concentration (MIC) using broth dilution and agar diffusion. In the part of the study supragingival dental plaque were obtained from 20 periodontally healthy adult volunteers. Descriptive analysis was done for the entire quantitative and qualitative variable recorded.
The MIC by broth dilution method found no statistically significant difference between the mouthwashes. The agar dilution method showed CHX was more effective as compared to the herbal mouthwash against standard strains of , , and . However, no difference was observed between the mouthwashes for , , and . The results conclude that none of the selected mouthwashes were statistically significantly different from each other.
In the present study, CHX showed higher levels of antimicrobial action than the herbal mouthwash against bacterial species. The results reinforce the earlier findings that the testing is sensitive to methods and due diligence is needed when extrapolating the data for further use. However, long-term use and effectiveness against the periopathogens need to be tested in well-planned clinical trials.
已对多种草本漱口水和草本提取物进行了测试，以寻找一种适合长期用于机械治疗的辅助剂。在本研究中，我们旨在观察草本漱口水和氯己定（CHX）漱口水对测试模型中选定微生物的抗菌效果。
测定了对参与牙周疾病不同阶段的标准菌株的抗菌效果。测试包括使用肉汤稀释法和琼脂扩散法测定最低抑菌浓度（MIC）。在研究的一部分中，从20名牙周健康的成年志愿者中获取龈上牙菌斑。对记录的所有定量和定性变量进行了描述性分析。
肉汤稀释法测定的MIC显示，漱口水之间无统计学显著差异。琼脂稀释法显示，与草本漱口水相比，CHX对标准菌株、和更有效。然而，对于、和，漱口水之间未观察到差异。研究结果得出结论，所选漱口水之间在统计学上均无显著差异。
在本研究中，CHX对细菌种类的抗菌作用水平高于草本漱口水。结果强化了早期的发现，即测试对方法敏感，在推断数据以供进一步使用时需要谨慎。然而，长期使用以及对牙周病原体的有效性需要在精心设计的临床试验中进行测试。
