Nepomniashchikh G I, Efremov V N, Tumanov V P, Nepomniashchikh L M
Biull Eksp Biol Med. 1986 Jan;101(1):105-9.
Cells obtained by bronchoalveolar lavage from patients with chronic nonspecific pulmonary inflammatory diseases were studied using light and electron microscopy and radioautography. Five morphological forms of alveolar macrophages, distinct in their structure and 3H-uridine content were described. A higher level of RNA synthesis has been revealed in alveolar macrophage forms 2 and 3 than in forms 1, 4 and 5; with it being lower in polymorphonuclear leukocytes and lymphocytes. It was shown that changes in the number of lavage cells and the structure-to-function characteristics in each cellular population depended on the phase of the inflammatory process. It was postulated that structural and metabolic heterogeneity of alveolar macrophages reflected the successive stages of cellular development from cell-precursors (through activation of protein synthesis) to cells with complete lysosomal cycle and the following phagocytosis.
采用光镜、电镜及放射自显影技术，对慢性非特异性肺炎患者支气管肺泡灌洗获得的细胞进行了研究。描述了肺泡巨噬细胞的五种形态学形式，它们在结构和3H-尿苷含量上各不相同。已发现肺泡巨噬细胞2型和3型的RNA合成水平高于1型、4型和5型；多形核白细胞和淋巴细胞中的RNA合成水平较低。结果表明，灌洗细胞数量的变化以及每个细胞群体的结构与功能特征取决于炎症过程的阶段。据推测，肺泡巨噬细胞的结构和代谢异质性反映了细胞从细胞前体（通过蛋白质合成激活）到具有完整溶酶体循环及随后吞噬作用的细胞的连续发育阶段。
