McDivitt R W, Stone K R, Craig R B, Palmer J O, Meyer J S, Bauer W C
Cancer. 1986 Jan 15;57(2):269-76. doi: 10.1002/1097-0142(19860115)57:2<269::aid-cncr2820570214>3.0.co;2-d.
Flow cytometric DNA analysis was performed on 168 consecutive primary operable breast cancers and the results correlated with thymidine labeling, estrogen receptor (ER) and progesterone receptor values, and various histologic parameters. Seventy-five cancers (45.0%) were diploid and 93 (55.0%) aneuploid. In 83.9% of aneuploid cancers, the DNA index fell between 1.1 and 2.0. Thirteen cancers were hypertetraploid and three hypodiploid. The percent of S-phase cancers (SpF) varied from 1.1% to 24.4%, with a mean of 8.0% and a median of 7.1%. The mean SpF of diploid cancers was 5.2%; of aneuploid cancers, 10.3%. There was no significant correlation between SpF or ploidy and tumor size or axillary lymph node status. The thymidine labeling index (TLI) varied from 0.2 to 23.1, with a mean of 7.5 and a median of 6.1. There was good correlation between TLI and SpF (r = 0.892, P = 0.0001). ER-negative tumors had a significantly higher mean SpF (10.3%) than did ER-positive tumors (6.7%), but there was no significant correlation between ploidy and receptor positivity or negativity. There was a good correlation between invasive tumor necrosis, poor cytologic differentiation, aneuploidy, and above-median SpF. Only a fair correlation was observed between mitotic rate and SpF. A classification of invasive breast cancers based on ploidy and SpF is proposed.
对168例连续的原发性可手术乳腺癌进行了流式细胞术DNA分析，并将结果与胸苷标记、雌激素受体（ER）和孕激素受体值以及各种组织学参数进行了关联。75例癌症（45.0%）为二倍体，93例（55.0%）为非整倍体。在83.9%的非整倍体癌症中，DNA指数在1.1至2.0之间。13例癌症为超四倍体，3例为亚二倍体。S期癌症百分比（SpF）从1.1%到24.4%不等，平均为8.0%，中位数为7.1%。二倍体癌症的平均SpF为5.2%；非整倍体癌症为10.3%。SpF或倍性与肿瘤大小或腋窝淋巴结状态之间无显著相关性。胸苷标记指数（TLI）从0.2到23.1不等，平均为7.5，中位数为6.1。TLI与SpF之间存在良好的相关性（r = 0.892，P = 0.0001）。ER阴性肿瘤的平均SpF（10.3%）显著高于ER阳性肿瘤（6.7%），但倍性与受体阳性或阴性之间无显著相关性。浸润性肿瘤坏死、细胞分化差、非整倍体和SpF高于中位数之间存在良好的相关性。仅观察到有丝分裂率与SpF之间存在中等程度的相关性。提出了一种基于倍性和SpF的浸润性乳腺癌分类方法。
