Zhang Junfeng, Gong Weiyi, Wang Xinle, Yang Longbo
Department of Thoracic Surgery, Zhongkegengjiu Hospital of Anhui/Gengjiu Clinical Medical College, Anhui Medical University, Hefei, 230001, China.
Iran J Public Health. 2024 Sep;53(9):2049-2058. doi: 10.18502/ijph.v53i9.16458.
Long non-coding RN (lncRNAs) have been implicated in lung cancer, but the mechanisms stay unclear. We investigated the theatrical role and mechanism of lncRNA Lung cancer associated transcript 1 in the malignant progress of lung cancer.
From May 2022 to March 2023, a total of thirty normal and cancerous tissues were collected from patients diagnosed with non-small cell lung cancer at Zhongke Gengjiu Hospital in Anhui Province, China. The human SPC-A1 and A549 cell lines were chosen as the subjects for the relevant cellular experiments in this study. LncRNAs were expressed in a different manner identified by bioinformatics methods, and the expression levels in lung cancer tissues as well as cells were verified by the qRT-PCR assay. The biological role of in NSCLC was determined by CCK-8, EdU, and transwell assay.
The regulation of ubiquitin of by was studied, which showed that was significantly elevated in NSCLC cell lines and patients' tissues (<0.05). High levels of promoted the proliferation, invasion, and migration of NSCLC cells. Mechanism studies showed that was mainly located in the nucleus, which bound to and mediated the ubiquitinated degradation of . Meanwhile, knockdown attenuated the ubiquitination process of . In addition, rescue experiments illustrated that induced the proliferation and invasion of NSCLC cells, and played a key role in the survival and tumorigenicity of NSCLC cells by mediating the ubiquitination of .
Collectively, activated the malignant phenotypes of NSCLC cells via regulating ubiquitination, which provided a new idea for the diagnosis and treatment of NSCLC.
长链非编码RNA（lncRNAs）与肺癌有关，但其机制尚不清楚。我们研究了lncRNA肺癌相关转录本1在肺癌恶性进展中的理论作用及机制。
2022年5月至2023年3月，从中国安徽省中科庚玖医院诊断为非小细胞肺癌的患者中收集了共30份正常组织和癌组织。本研究选择人SPC-A1和A549细胞系作为相关细胞实验的对象。通过生物信息学方法鉴定lncRNAs的不同表达方式，并通过qRT-PCR检测验证其在肺癌组织及细胞中的表达水平。通过CCK-8、EdU和transwell实验确定其在非小细胞肺癌中的生物学作用。
研究了[具体内容]对[另一具体内容]泛素化的调控，结果显示其在非小细胞肺癌细胞系和患者组织中显著升高（<0.05）。高水平的[具体内容]促进了非小细胞肺癌细胞的增殖、侵袭和迁移。机制研究表明，[具体内容]主要位于细胞核中，它与[另一具体内容]结合并介导了[另一具体内容]的泛素化降解。同时，[具体内容]敲低减弱了[另一具体内容]的泛素化过程。此外，拯救实验表明，[具体内容]诱导了非小细胞肺癌细胞的增殖和侵袭，并通过介导[另一具体内容]的泛素化在非小细胞肺癌细胞的存活和致瘤性中起关键作用。
总体而言，[具体内容]通过调节[另一具体内容]的泛素化激活了非小细胞肺癌细胞的恶性表型，这为非小细胞肺癌的诊断和治疗提供了新思路。
