使用GutHi-C高效且易于操作地捕获三维宏基因组相互作用。

Efficient and easy-to-use capturing three-dimensional metagenome interactions with GutHi-C.

作者信息

Lu Yu-Xi, Yang Jin-Bao, Li Chen-Ying, Tian Yun-Han, Chang Rong-Rong, Kong Da-Shuai, Yang Shu-Lin, Wang Yan-Fang, Zhang Yu-Bo, Zhu Xiu-Sheng, Pan Wei-Hua, Kong Si-Yuan

机构信息

Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi-Omics of MARA, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen China.

School of Life Sciences Henan University Kaifeng China.

出版信息

Imeta. 2024 Jul 22;3(5):e227. doi: 10.1002/imt2.227. eCollection 2024 Oct.

DOI:10.1002/imt2.227

PMID:39429879

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11487548/

Abstract

Hi-C can obtain three-dimensional chromatin structure information and is widely used for genome assembly. We constructed the GutHi-C technology. As shown in the graphical abstract, it is a highly efficient and quick-to-operate method and can be widely used for human, livestock, and poultry gut microorganisms. It provides a reference for the Hi-C methodology of the microbial metagenome. DPBS, Dulbecco's phosphate-buffered saline; Hi-C, high-through chromatin conformation capture; LB, Luria-Bertani; NGS, next-generation sequencing; PCR, polymerase chain reaction; QC, quality control.

摘要

Hi-C技术能够获取三维染色质结构信息，被广泛应用于基因组组装。我们构建了GutHi-C技术。如图形摘要所示，它是一种高效且易于操作的方法，可广泛应用于人类、家畜和家禽肠道微生物。它为微生物宏基因组的Hi-C方法提供了参考。DPBS，杜氏磷酸盐缓冲盐水；Hi-C，高通量染色质构象捕获；LB，Luria-Bertani培养基；NGS，下一代测序；PCR，聚合酶链反应；QC，质量控制。