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性侵转介中心(SARC)内用于DNA证据采集的耗材验证。

Validation of consumables used in the recovery of DNA evidence within Sexual Assault Referral Centres (SARCs).

作者信息

Gaskell Michelle, Guiness June, Sullivan Kevin

机构信息

Forensic Capability Network, Dorset Police, Dorset, UK.

University of Central Lancashire, UK.

出版信息

Forensic Sci Int Synerg. 2024 Oct 8;9:100559. doi: 10.1016/j.fsisyn.2024.100559. eCollection 2024.

DOI:10.1016/j.fsisyn.2024.100559
PMID:39429977
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11490862/
Abstract

This study assessed the fitness for purpose of consumables used for the recovery of body-fluids and biological material for subsequent DNA analysis in forensic medical examinations, specifically within Sexual Assault Referral Centres (SARCs). All 55 SARCs in England and Wales participated in this validation exercise by submitting unused critical DNA consumables from their local storage areas. Overall, 261 consumable items identified as forensic DNA grade were assessed for the presence of DNA contamination and 100 % passed testing, demonstrating that consumables purchased under the National Scenes of Crimes Consumables Contract remained fit for purpose, regarding detectable levels of DNA, up to the point of use within the SARCs. In addition, 14 non-forensic DNA grade items were analysed, of which over a third failed due to unsourced DNA contamination, highlighting why it is essential that only forensic DNA grade consumables are used for evidential recovery processes to minimise the risk of contamination.

摘要

本研究评估了用于在法医学检查中,特别是在性侵犯转介中心(SARC)内采集体液和生物材料以供后续DNA分析的消耗品是否符合使用目的。英格兰和威尔士的所有55个SARC都参与了此次验证工作,它们从当地储存区域提交了未使用过的关键DNA消耗品。总体而言,对261件被认定为法医DNA级别的消耗品进行了DNA污染检测评估,所有消耗品100%通过测试,这表明根据《国家犯罪现场消耗品合同》购买的消耗品,就可检测到的DNA水平而言,在SARC内使用前仍符合使用目的。此外,对14件非法医DNA级别的物品进行了分析,其中超过三分之一因来源不明的DNA污染而不合格,这凸显了为何在证据采集过程中必须仅使用法医DNA级别的消耗品,以将污染风险降至最低。

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本文引用的文献

1
Validation of a dual cycle ethylene oxide treatment technique to remove DNA from consumables used in forensic laboratories.验证一种双循环环氧乙烷处理技术,以去除法医实验室中使用的消耗品中的 DNA。
Forensic Sci Int Genet. 2010 Jul;4(4):239-43. doi: 10.1016/j.fsigen.2009.10.004. Epub 2009 Nov 14.
2
Manufacturer contamination of disposable plastic-ware and other reagents--an agreed position statement by ENFSI, SWGDAM and BSAG.一次性塑料制品及其他试剂的制造商污染——欧洲法医科学研究所网络(ENFSI)、美国法医DNA分析科学工作组(SWGDAM)和英国法医科学咨询小组(BSAG)的一致立场声明
Forensic Sci Int Genet. 2010 Jul;4(4):269-70. doi: 10.1016/j.fsigen.2009.08.009. Epub 2009 Sep 6.
3
New developments and challenges in the use of the UK DNA Database: addressing the issue of contaminated consumables.英国DNA数据库使用中的新进展与挑战:解决消耗品污染问题
Forensic Sci Int. 2004 Dec 2;146 Suppl:S175-6. doi: 10.1016/j.forsciint.2004.09.053.