Research and Development, Forensic Science Service Ltd. 2960 Trident Court Solihull Parkway, Birmingham Business Park, Birmingham, United Kingdom.
Forensic Sci Int Genet. 2010 Jul;4(4):239-43. doi: 10.1016/j.fsigen.2009.10.004. Epub 2009 Nov 14.
Validation of a dual cycle ethylene oxide (EO) approach to removing DNA contamination from consumables has been undertaken. The limits of the technique were investigated resulting in evidence that the DNA from up to 50microl of blood and saliva can be removed to a level where the consumable can be considered DNA free. DNA from semen was more resilient and some allelic peaks remained that would have prevented the consumable being classed as suitable for use in low template DNA analysis. No residual effect on consumables resulting in inhibition of subsequent DNA analysis was noted. However, if consumables had been previously treated with gamma or electron beam irradiation then slight inhibition of the downstream STR process was observed. Dual cycle EO treatment was effective at removing recoverable DNA from swabs and stain cards and consideration should be given to the suitability of EO treatment for use on critical consumables used in the forensic laboratory.
已经对双循环环氧乙烷 (EO) 去除耗材中 DNA 污染的方法进行了验证。研究了该技术的极限，结果表明，多达 50μl 的血液和唾液中的 DNA 可以被去除到耗材可以被认为是无 DNA 的水平。来自精液的 DNA 更有弹性，仍有一些等位基因峰存在，这将阻止耗材被归类为适合用于低模板 DNA 分析。没有观察到对耗材的残留影响，从而抑制了后续的 DNA 分析。然而，如果耗材以前曾用γ或电子束辐照处理过，则下游 STR 过程会受到轻微抑制。双循环 EO 处理可有效去除拭子和污渍卡上可回收的 DNA，应考虑将 EO 处理用于法医实验室中使用的关键耗材。
