Claësson M H
Scand J Immunol. 1979;10(4):339-42. doi: 10.1111/j.1365-3083.1979.tb01360.x.
Non-adherent spleen cells from athymic nude mice showed incorporation of tritiated thymidine (3H-TdR) at a level comparable to that of non-adherent cells of normal mice when stimulated with the T-cell mitogen phytohaemagglutinin (PHA). The optimal dose of PHA was 50 microliter/ml for nude cells, a concentration that does not stimulate normal cells. In comparison, 2.5 microliter/ml PHA was optimal for cells of normal mice. Anti-theta serum plus complement treatment of the non-adherent nude spleen cells reduced 3H-TdR incorporation by approximately 50%, suggesting that some of the responding cells carried the theta antigen. Direct immunofluorescence showed that the non-adherent nude spleen cells contained 6-9% theta-positive cells. In comparison, more than 80% of the PHA-induced nude blast cells were theta-positive. PHA-induced DNA synthesis of normal mouse spleen cells was inhibited by adherent nude spleen cells. No theta-antigen or surface immunoglobulin could be demonstrated on these inhibitory cells, suggesting that they belong to the macrophage system of the nude mouse spleen.
用T细胞促有丝分裂原植物血凝素（PHA）刺激时，无胸腺裸鼠的非黏附性脾细胞掺入氚标记胸腺嘧啶核苷（3H-TdR）的水平与正常小鼠的非黏附性细胞相当。PHA对裸鼠细胞的最佳剂量为50微升/毫升，该浓度不会刺激正常细胞。相比之下，2.5微升/毫升的PHA对正常小鼠细胞是最佳的。用抗θ血清加补体处理非黏附性裸脾细胞可使3H-TdR掺入减少约50%，这表明一些反应细胞带有θ抗原。直接免疫荧光显示非黏附性裸脾细胞含有6-9%的θ阳性细胞。相比之下，超过80%的PHA诱导的裸鼠母细胞是θ阳性的。正常小鼠脾细胞的PHA诱导的DNA合成受到黏附性裸脾细胞的抑制。在这些抑制细胞上未检测到θ抗原或表面免疫球蛋白，这表明它们属于裸鼠脾的巨噬细胞系统。
