Claësson M H, Hansen G S, Tjell V
Med Microbiol Immunol. 1979 Aug;167(3):161-74. doi: 10.1007/BF02121182.
The effect of D-penicillamine (D-Pen) on the proliferation of cultures of normal mouse, rat, and human spleen lymphocytes and peripheral blood lymphocytes was examined. D-Pen in concentrations of 2 x 10(-3) M to 8 x 10(-3) M in serum-free and in serum-containing medium resulted in a highly significant incorporation of 3H-TdR by normal mouse and rat spleen cells. Enhanced incorporation of 3H-TdR by normal human spleen cells only occurred in serum-containing medium. D-Pen in concentrations of 10(-4) M to 10(-3) M in serum-free and serum-containing medium resulted in significant inhibition of 3H-TdR incorporation by normal and mitogen-stimulated mouse and rat spleen cells. Doses of D-Pen greater than 2 x 10(-2) M strongly inhibited 3H-TdR incorporation by both normal and mitogen-stimulated mouse, rat, and human spleen cells and peripheral blood cells. The latter cells were not stimulated or inhibited at lower concentrations of D-Pen. Results from cell depletion and enriching procedures (specific antibody +C' cell killing, employment of athymic, nude spleen cells, adherent and phagocytic cell removal, E rosette cell separation procedures) suggested that target cells in the mouse spleen for D-Pen activation are non-adherent B cells whereas the D-Pen responsive cells in the human spleen probably are T cells.
研究了D-青霉胺（D-Pen）对正常小鼠、大鼠和人脾淋巴细胞及外周血淋巴细胞培养物增殖的影响。在无血清和含血清培养基中，浓度为2×10⁻³M至8×10⁻³M的D-Pen导致正常小鼠和大鼠脾细胞对³H-TdR的掺入率显著升高。正常人类脾细胞对³H-TdR掺入率的增强仅发生在含血清培养基中。在无血清和含血清培养基中，浓度为10⁻⁴M至10⁻³M的D-Pen导致正常和经丝裂原刺激的小鼠和大鼠脾细胞对³H-TdR掺入的显著抑制。剂量大于2×10⁻²M的D-Pen强烈抑制正常和经丝裂原刺激的小鼠、大鼠和人脾细胞及外周血细胞对³H-TdR的掺入。在较低浓度的D-Pen下，后一类细胞未受到刺激或抑制。细胞耗竭和富集程序（特异性抗体+C'细胞杀伤、使用无胸腺裸鼠脾细胞、去除黏附细胞和吞噬细胞、E花环细胞分离程序）的结果表明，小鼠脾中D-Pen激活的靶细胞是非黏附性B细胞，而人脾中对D-Pen有反应的细胞可能是T细胞。
