Dib Israa, Noureddine Hiba, Fakih Mohamad, Livet Alexandre, Alphonse Vanessa, Illayk Abbas, Medlej Abdallah Ahmad, Tarhini Mahdi, Bousserrhine Noureddine
Laboratory of Water Environment and Urban Systems, University Paris-Est Créteil, Créteil Cedex, France.
Environmental Health Research Laboratory, Faculty of Sciences V, Lebanese University, Nabatieh, Lebanon.
J Interferon Cytokine Res. 2024 Dec;44(12):550-556. doi: 10.1089/jir.2024.0183. Epub 2024 Oct 4.
Toll-like receptors (TLRs), particularly the TLR2, take part in the elicitation of immune responses against . This study aimed to investigate the relationship between a specific polymorphism called rs3804099 in the gene and infection among Lebanese children. A case-control study encompassed 180 participants including 68 children with amebiasis and 112 matched controls. Blood samples were collected, and genomic DNA was extracted using the classical proteinase K digestion and phenol-chloroform extraction method. The variant rs3804099 was examined using the Amplification Refractory Mutation System Polymerase Chain Reaction. The accuracy of the genotyping was supported by sequencing 5% of samples. The rs3804099 polymorphism was identified in the studied population, and the observed genotypic distributions were consistent with equilibrium ( > 0.05). The frequency of the rare CC genotype was significantly higher in patients compared to the noninfected group ( < 0.01). In controls, the homozygous TT genotype was less frequent than the heterozygous CT genotype. The rare CC genotype was associated with a higher risk of amebiasis among children (odds ratios = 3.27, = 0.002). These findings provide evidence supporting the association between the rs3804099 SNP in the gene and infection among Lebanese children.
Toll样受体（TLRs），尤其是TLR2，参与引发针对……的免疫反应。本研究旨在调查……基因中一种名为rs3804099的特定多态性与黎巴嫩儿童……感染之间的关系。一项病例对照研究纳入了180名参与者，其中包括68名患阿米巴病的儿童和112名匹配的对照。采集血样，采用经典的蛋白酶K消化和酚-氯仿提取法提取基因组DNA。使用扩增阻滞突变系统聚合酶链反应检测rs3804099变体。通过对5%的样本进行测序来支持基因分型的准确性。在所研究的人群中鉴定出了rs3804099多态性，观察到的基因型分布符合……平衡（>0.05）。与未感染组相比，患者中罕见的CC基因型频率显著更高（<0.01）。在对照组中，纯合子TT基因型的频率低于杂合子CT基因型。罕见的CC基因型与儿童患阿米巴病的较高风险相关（优势比=3.27，=0.002）。这些发现提供了证据，支持……基因中的rs3804099单核苷酸多态性与黎巴嫩儿童……感染之间的关联。
