Hultmark D, Klemenz R, Gehring W J
Cell. 1986 Feb 14;44(3):429-38. doi: 10.1016/0092-8674(86)90464-2.
Downstream of the transcription start site in the Drosophila heat-shock gene hsp22, we have identified a region that is necessary for efficient transcription, and also for selective translation during heat shock. We assayed the expression of mutated genes after P-factor-mediated insertion into the genome. Deletions within the first 26 nucleotides block the preferential translation of hsp22 mRNA at high temperature. The rate of transcription is also decreased, though transcription is still heat-inducible. Leaving this region intact, up to 86% of the leader can be deleted without affecting translation or transcription. The functional region coincides with a region of sequence homology between the heat-shock genes. Only partial homology is found to a conserved sequence, ATCAGTTCT, found at the very 5' end of other insect genes.
在果蝇热休克基因hsp22转录起始位点的下游，我们鉴定出一个区域，该区域对于高效转录以及热休克期间的选择性翻译都是必需的。我们检测了P因子介导插入基因组后突变基因的表达。前26个核苷酸内的缺失会阻断hsp22 mRNA在高温下的优先翻译。转录速率也会降低，尽管转录仍然是热诱导的。保留该区域完整，多达86%的前导序列可以被删除而不影响翻译或转录。该功能区域与热休克基因之间的序列同源区域一致。在其他昆虫基因的5'端发现的保守序列ATCAGTTCT只存在部分同源性。
