Klemenz R, Hultmark D, Gehring W J
EMBO J. 1985 Aug;4(8):2053-60. doi: 10.1002/j.1460-2075.1985.tb03891.x.
One of the effects of a temperature increase above 35 degrees C on Drosophila melanogaster is a rapid switch in selectivity of the translational apparatus. Protein synthesis from normal, but not from heat shock, mRNA is much reduced. Efficient translation at high temperature might be a result of the primary sequence of heat shock genes. Alternatively a mRNA modification mechanism, altered as a consequence of heat shock, might allow for efficient high temperature translation of any mRNA synthesized during a heat shock. The gene for alcohol dehydrogenase (Adh) was fused to the controlling elements of a heat shock protein 70 (hsp70) gene. Authentic Adh mRNA, synthesized from this fusion gene at elevated temperatures was not translated during heat shock. A second Adh fusion gene in which the mRNA synthesized contained the first 95 nucleotides of the Hsp70 non-translated leader sequence gave rise, at high temperature, to mRNA which was translated during the heat shock. Thus, the signal(s) in the mRNAs controlling translation efficiency at heat shock temperatures is encoded within the heat shock genes.
温度升高至35摄氏度以上对黑腹果蝇的影响之一是翻译装置的选择性迅速转变。正常mRNA的蛋白质合成大幅减少，而热休克mRNA的蛋白质合成则不然。高温下的高效翻译可能是热休克基因一级序列的结果。或者，热休克导致改变的mRNA修饰机制可能允许热休克期间合成的任何mRNA在高温下高效翻译。乙醇脱氢酶（Adh）基因与热休克蛋白70（hsp70）基因的控制元件融合。在高温下从该融合基因合成的真实Adh mRNA在热休克期间未被翻译。第二个Adh融合基因合成的mRNA包含Hsp70非翻译前导序列的前95个核苷酸，在高温下产生的mRNA在热休克期间被翻译。因此，热休克温度下控制翻译效率的mRNA中的信号是由热休克基因编码的。
