School of Integrative Engineering, Chung-Ang University.
School of Integrative Engineering, Chung-Ang University; Feynman Institute of Technology, Nanomedicine Corporation; Department of Chemical Science and Engineering, Tokyo Institute of Technology.
J Vis Exp. 2024 Oct 4(212). doi: 10.3791/66762.
Antibiotics are currently the most used antibacterial treatment for killing bacteria. However, bacteria develop resistance when continually overexposed to antibiotics. Developing antimicrobial agents that can replace existing antibiotics is essential because antibiotic-resistant bacteria have resistance mechanisms for all current antibiotics and can promote nosocomial infections. To address this challenge, in this study, we propose graphene oxide/copper (GO/Cu) nanocomposites as antibacterial materials that can replace the existing antibiotics. GO/Cu nanocomposites are characterized by transmission electron microscopy and scanning electron microscopy. They show that copper (Cu) nanoparticles are well-grown on the graphene oxide sheets. Additionally, a microdilution broth method is used to confirm the efficacy of the antimicrobial substance against methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa (P. aeruginosa), which are frequently implicated in nosocomial infections. Specifically, 99.8% of MRSA and 84.7% of P. aeruginosa are eliminated by 500 µg/mL of GO/Cu nanocomposites. Metal nanocomposites can eradicate antibiotic-resistant bacteria by releasing ions, forming reactive oxygen species, and physically damaging the bacteria. This study demonstrates the potential of antibacterial GO/Cu nanocomposites in eradicating antibiotic-resistant bacteria.
抗生素是目前用于杀灭细菌的最常用抗菌治疗方法。然而，当细菌持续过度暴露于抗生素时，它们会产生耐药性。开发可以替代现有抗生素的抗菌剂至关重要，因为具有耐药机制的抗生素耐药菌对所有现有抗生素都具有耐药性，并可能促进医院感染。为了解决这一挑战，在本研究中，我们提出了氧化石墨烯/铜（GO/Cu）纳米复合材料作为可替代现有抗生素的抗菌材料。GO/Cu 纳米复合材料的特点是通过透射电子显微镜和扫描电子显微镜进行观察。结果表明，铜（Cu）纳米颗粒在氧化石墨烯片上生长良好。此外，还使用微量稀释肉汤法来确认抗菌物质对耐甲氧西林金黄色葡萄球菌（MRSA）和铜绿假单胞菌（P. aeruginosa）的功效，这两种细菌经常与医院感染有关。具体而言，GO/Cu 纳米复合材料的浓度为 500μg/mL 时，可以消除 99.8%的 MRSA 和 84.7%的 P. aeruginosa。金属纳米复合材料可以通过释放离子、形成活性氧物质和物理损伤细菌来根除抗生素耐药菌。本研究表明了抗菌 GO/Cu 纳米复合材料在根除抗生素耐药菌方面的潜力。
