Jang Jaehee, Lee Jong-Min, Oh Sang-Bin, Choi Yonghyun, Jung Han-Sung, Choi Jonghoon
School of Integrative Engineering, Chung-Ang University, Seoul 06974, Republic of Korea.
Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul 03722, Republic of Korea.
ACS Appl Mater Interfaces. 2020 Aug 12;12(32):35826-35834. doi: 10.1021/acsami.0c06054. Epub 2020 Jul 28.
There are numerous issues associated with bacteria, particularly biofilms, which exhibit a strong resistance to antibiotics. This is currently considered an urgent global issue owing to the lack of effective treatments. Graphene oxide (GO) nanosheets are two-dimensional carbon materials that are available as a substrate for metal nanoparticles and have a lower release rate of metal ions than free metal nanoparticles by regulating the oxidation of metal nanoparticles, which is known to reduce the cytotoxicity caused by the free metal nanoparticles. Over centuries, metal particles, including Ag and Cu, have been considered as antibacterial agents. In this study, Ag and Cu bimetallic nanoparticles on a GO surface (Ag/Cu/GO) were synthesized using a chemical reduction method, and their antimicrobial effects against several bacterial species were demonstrated. Ag/Cu/GO nanocomposites were characterized by transmission electron microscopy and energy-dispersive X-ray spectroscopy. The in vitro cytotoxicity of an Ag/Cu/GO nanocomposite was evaluated in human dermal fibroblasts, and its antibacterial activity against , ., and () was also tested. The synthesized Ag/Cu/GO nanocomposite was able to eradicate all three bacterial species at a concentration that was harmless to human cells. In addition, Ag/Cu/GO successfully removed a biofilm originated from the culturing of in a microchannel with a dynamic flow. In a small-animal model, a biofilm-infected skin wound was healed quickly and efficiently by the topical application of Ag/Cu/GO. The Ag/Cu/GO nanocomposites reported in this study could be used to effectively remove antibiotic-resistant bacteria and treat diseases in the skin or wound due to bacterial infections and biofilm formation.
细菌存在诸多问题，尤其是生物膜，其对抗生素表现出很强的抗性。由于缺乏有效的治疗方法，这目前被视为一个紧迫的全球问题。氧化石墨烯（GO）纳米片是二维碳材料，可作为金属纳米颗粒的基底，并且通过调节金属纳米颗粒的氧化，其金属离子释放速率比游离金属纳米颗粒低，已知这可降低游离金属纳米颗粒引起的细胞毒性。几个世纪以来，包括银和铜在内的金属颗粒一直被视为抗菌剂。在本研究中，采用化学还原法合成了GO表面的银和铜双金属纳米颗粒（Ag/Cu/GO），并证明了它们对几种细菌的抗菌效果。通过透射电子显微镜和能量色散X射线光谱对Ag/Cu/GO纳米复合材料进行了表征。在人皮肤成纤维细胞中评估了Ag/Cu/GO纳米复合材料的体外细胞毒性，并测试了其对金黄色葡萄球菌、大肠杆菌及绿脓杆菌（铜绿假单胞菌）的抗菌活性。合成的Ag/Cu/GO纳米复合材料能够在对人体细胞无害的浓度下根除所有这三种细菌。此外，Ag/Cu/GO成功去除了微通道中动态流动培养的金黄色葡萄球菌形成的生物膜。在一个小动物模型中，通过局部应用Ag/Cu/GO，生物膜感染的皮肤伤口快速且有效地愈合。本研究报道的Ag/Cu/GO纳米复合材料可用于有效去除耐抗生素细菌，并治疗因细菌感染和生物膜形成导致的皮肤或伤口疾病。
