Clark D R, Inloes R L, Kalman S M, Sussman H H
Clin Chem. 1986 Feb;32(2):381-5.
Automated digoxin immunoassays with the Abbott TDx, Dade Stratus, and Du Pont aca were evaluated against an RIA similar to the Centers for Disease Control Candidate Reference Method. All three automated methods correlated poorly with the reference method. Particularly disconcerting were the falsely increased values (up to 0.6 micrograms/L) obtained by the Stratus for blank specimens. We discuss the inaccuracies of the results in terms of sample matrix effects, calibrator problems, lot-to-lot reagent variation, and our laboratory requirements. We strongly support the acceptance of the CDC Candidate Reference Method for digoxin and the establishment of a standardized protocol for determining the accuracy of digoxin measurements.
对使用雅培TDx、达德Stratus和杜邦aca的自动化地高辛免疫测定法与一种类似于疾病控制中心候选参考方法的放射免疫分析法（RIA）进行了评估。所有这三种自动化方法与参考方法的相关性都很差。特别令人不安的是，Stratus对空白样本得出的错误升高值（高达0.6微克/升）。我们从样本基质效应、校准物问题、批次间试剂差异以及我们实验室的要求等方面讨论了结果的不准确之处。我们强烈支持采用疾病控制中心的地高辛候选参考方法，并建立一个用于确定地高辛测量准确性的标准化方案。
