Leflar C C, Freytag J W, Powell L M, Strahan J C, Wadsley J J, Tyler C A, Miller W K
Clin Chem. 1984 Nov;30(11):1809-11.
We describe an automated assay for digoxin that requires a 200-microL sample of serum. Total analysis time is 18 min. The method is extremely precise, with within-run CVs of 2.6, 1.6, and 4.9%, respectively, at 0.5, 1.5, and 3.5 micrograms of digoxin per liter (n = 20). The lower limit of detection is 0.2 micrograms of digoxin per liter. For patients' samples, the correlation with RIA (x) is excellent (r = 0.95; y = 0.95x - 0.14; standard error = 0.17 micrograms/mL). We saw no interferences in samples having high concentrations of rheumatoid factors, lipid, bilirubin, or hemoglobin. Cross reactivity with digoxin analogs and steroidal compounds is similar to that observed by RIA.
我们描述了一种地高辛自动化检测方法，该方法需要200微升血清样本。总分析时间为18分钟。该方法极其精确，在每升含0.5、1.5和3.5微克地高辛时，批内变异系数分别为2.6%、1.6%和4.9%（n = 20）。检测下限为每升0.2微克地高辛。对于患者样本，与放射免疫分析（RIA）（x）的相关性极佳（r = 0.95；y = 0.95x - 0.14；标准误差 = 0.17微克/毫升）。我们在类风湿因子、脂质、胆红素或血红蛋白浓度高的样本中未发现干扰。与地高辛类似物和甾体化合物的交叉反应性与放射免疫分析所观察到的相似。
