Isaac Sharleen Livina, Mohd Hashim Amalia, Faizal Wong Fadzlie Wong, Mohamed Akbar Muhamad Afiq, Wan Ahmad Kamil Wan Nur Ismah
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia.
Halal Products Research Institute, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia.
Probiotics Antimicrob Proteins. 2025 Jun;17(3):937-962. doi: 10.1007/s12602-024-10384-3. Epub 2024 Oct 21.
Lactic acid bacteria (LAB) are widely known for the production of secondary metabolites such as organic acids and other bioactive compounds such as bacteriocins. Finding a broad application in food and healthcare, bacteriocins have received increased attention due to their inherent antimicrobial properties. However, the extraction of bacteriocins is often plagued with low yields due to the complexity of the extraction processes and the diversity of bacteriocins themselves. Here, we review the current knowledge related to bacteriocin extraction on the different extraction techniques for isolating bacteriocins from LAB. The advantages and disadvantages of each technique will also be critically appraised, taking into account factors such as extraction efficiency, scalability and cost-effectiveness. This review aims to guide researchers and professionals in selecting the most suitable approach for bacteriocin extraction from LAB by illuminating the respective advantages and limitations of various extraction techniques.
乳酸菌（LAB）以产生有机酸等次生代谢产物以及细菌素等其他生物活性化合物而广为人知。细菌素因其固有的抗菌特性而在食品和医疗保健领域得到广泛应用，受到了越来越多的关注。然而，由于提取过程的复杂性以及细菌素本身的多样性，细菌素的提取往往产量较低。在此，我们综述了目前与从乳酸菌中分离细菌素的不同提取技术相关的知识。每种技术的优缺点也将得到批判性评估，同时考虑提取效率、可扩展性和成本效益等因素。本综述旨在通过阐明各种提取技术的各自优势和局限性，指导研究人员和专业人员选择从乳酸菌中提取细菌素的最合适方法。
