Shentu Huifei, Ye Pengxin, Zhou Qingqing, Li Ping, Gu Qing
College of Food Science and Biotechnology, Zhejiang Gongshang University, Key Laboratory for Food Microbial Technology of Zhejiang Province, Hangzhou, Zhejiang, 310018, China.
Biochem Biophys Rep. 2023 Jul 10;35:101494. doi: 10.1016/j.bbrep.2023.101494. eCollection 2023 Sep.
Bacteriocins from lactic acid bacteria (LAB) have attracted widespread attention as a new type of biological preservative due to their safety, high efficiency, and non-toxic characteristics. In this study, we focused on Sakacin ZFM225, a novel bacteriocin produced by ZFM225, which was isolated from raw milk. It was purified by a strategy including precipitation with 70% ammonium sulfate, cation exchange chromatography and reverse-phase high performance liquid chromatography (RP-HPLC). The predicted molecular weight of Sakacin ZFM225 was 14950.92 Da. Sakacin ZFM225 exhibited resistance to high temperatures, strong activity under acidic conditions, and sensitivity to trypsin and pepsin. Bacteriocins from mainly inhibited the growth of . The bacteriocin possessed a broad-spectrum inhibition which could kill many foodborne pathogens such as , , and . We further demonstrated that the mode of action of Sakacin ZFM225 was related to the formation of cell membrane porosity, and excluded Lipid Ⅱ as its target. These results suggest that this new bacteriocin has great potential in food industry as a biological preservative and even medical field.
来自乳酸菌（LAB）的细菌素因其安全、高效、无毒的特性，作为一种新型生物防腐剂受到了广泛关注。在本研究中，我们聚焦于从生牛奶中分离出的ZFM225所产生的新型细菌素Sakacin ZFM225。它通过包括70%硫酸铵沉淀、阳离子交换色谱和反相高效液相色谱（RP-HPLC）在内的策略进行纯化。Sakacin ZFM225的预测分子量为14950.92道尔顿。Sakacin ZFM225表现出耐高温、在酸性条件下活性强以及对胰蛋白酶和胃蛋白酶敏感的特性。来自[具体来源未明确]的细菌素主要抑制[具体抑制对象未明确]的生长。该细菌素具有广谱抑制作用，可杀死许多食源性病原体，如[具体病原体未明确列举]。我们进一步证明Sakacin ZFM225的作用模式与细胞膜孔隙的形成有关，并且排除了脂磷壁酸前体Ⅱ作为其作用靶点。这些结果表明，这种新型细菌素作为生物防腐剂在食品工业甚至医学领域具有巨大潜力。
