Cheruvari Athira, Kammara Rajagopal
Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysore, 570020, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
Probiotics Antimicrob Proteins. 2024 Oct 21. doi: 10.1007/s12602-024-10378-1.
The aim of the study was the preliminary genetic and phenotypic characterization of a potential probiotic strain of Lactiplantibacillus pentosus (strain krglsrbmofpi2) obtained from traditionally fermented rice. Genome sequencing revealed that the strain has a 3.7-Mb genome with a GC content of 46 and a total of 3192 protein-coding sequences. Using bioinformatic methods, we have successfully identified phage genes, plasmids, pathogenicity, antibiotic resistance and a variety of bacteriocins. Through comprehensive biochemical and biophysical analyses, we have gained valuable insights into its auto-aggregation, co-aggregation, antibiotic resistance, hydrophobicity, antioxidant activity and tolerance to simulated gastrointestinal conditions. The safety evaluation of the isolated L. pentosus was performed on the basis of its haemolytic activity. Our studies have shown that this strain has a strong antagonistic activity against the priority pathogens identified by the World Health Organization such as Vibrio cholerae, Clostridium perfringens, Salmonella enterica subsp. enterica ser. Typhi, Escherichia coli, Listeria monocytogenes and Staphylococcus aureus. It is essential to fully understand the genetic and functional properties of the L. pentosus strain before considering its use as a useful probiotic in the food industry.
本研究的目的是对从传统发酵大米中获得的戊糖乳杆菌潜在益生菌菌株（菌株krglsrbmofpi2）进行初步的遗传和表型特征分析。基因组测序显示，该菌株有一个3.7兆碱基的基因组，GC含量为46%，共有3192个蛋白质编码序列。使用生物信息学方法，我们成功鉴定了噬菌体基因、质粒、致病性、抗生素抗性和多种细菌素。通过全面的生化和生物物理分析，我们对其自聚集、共聚集、抗生素抗性、疏水性、抗氧化活性以及对模拟胃肠道条件的耐受性有了宝贵的认识。基于其溶血活性对分离出的戊糖乳杆菌进行了安全性评估。我们的研究表明，该菌株对世界卫生组织确定的重点病原体如霍乱弧菌、产气荚膜梭菌、肠道沙门氏菌肠炎亚种伤寒血清型、大肠杆菌、单核细胞增生李斯特菌和金黄色葡萄球菌具有很强的拮抗活性。在考虑将戊糖乳杆菌菌株用作食品工业中的有益益生菌之前，充分了解其遗传和功能特性至关重要。
