Stergiou Odysseas Sotirios, Tegopoulos Konstantinos, Kiousi Despoina Eugenia, Tsifintaris Margaritis, Papageorgiou Aristotelis C, Tassou Chrysoula C, Chorianopoulos Nikos, Kolovos Petros, Galanis Alex
Department of Molecular Biology and Genetics, Faculty of Health Sciences, Democritus University of Thrace, Alexandroupolis, Greece.
Institute of Technology of Agricultural Products, Hellenic Agricultural Organization DIMITRA, Athens, Greece.
Front Microbiol. 2021 Oct 26;12:746659. doi: 10.3389/fmicb.2021.746659. eCollection 2021.
is a diverse genus that includes species of industrial and biomedical interest. , formerly known as , is a recently reclassified species, that contains strains isolated from diverse environmental niches, ranging from fermented products to mammalian gut microbiota. Importantly, several strains present health-promoting properties, such as immunomodulatory and antiproliferative activities, and are regarded as potential probiotic strains. In this study, we present the draft genome sequence of the potential probiotic strain L33, originally isolated from fermented sausages. Comprehensive bioinformatic analysis and whole-genome annotation were performed to highlight the genetic loci involved in host-microbe interactions and the probiotic phenotype. Consequently, we found that this strain codes for bile salt hydrolases, adhesins and moonlighting proteins, and for Class IIb bacteriocin peptides lacking the GxxxG and GxxxG-like motifs, crucial for their inhibitory activity. Its adhesion ability was also validated , on human cancer cells. Furthermore, L33 contains an exopolysaccharide (EPS) biosynthesis cluster, and it does not carry transferable antibiotic resistance genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and CAZymes analyses showed that L33 possesses biosynthetic pathways for seven amino acids, while it can degrade a wide array of carbohydrates. In parallel, Clusters of Orthologous Groups (COGs) and KEGG profiles of L33 are similar to those of 26 strains, as well as of two well documented probiotic strains. Conclusively, L33 exhibits good probiotic potential, although further studies are needed to elucidate the extent of its biological properties.
是一个多样化的属，包括具有工业和生物医学意义的物种。 ，以前称为 ，是一个最近重新分类的物种，包含从不同环境生态位分离的菌株，范围从发酵产品到哺乳动物肠道微生物群。重要的是，几种 菌株具有促进健康的特性，如免疫调节和抗增殖活性，被视为潜在的益生菌菌株。在本研究中，我们展示了潜在益生菌菌株 L33 的基因组草图序列，该菌株最初从发酵香肠中分离出来。进行了全面的生物信息学分析和全基因组注释，以突出参与宿主 - 微生物相互作用和益生菌表型的基因座。因此，我们发现该菌株编码胆盐水解酶、黏附素和兼职蛋白，以及缺乏对其抑制活性至关重要的 GxxxG 和 GxxxG 样基序的 IIb 类细菌素肽。其在人癌细胞上的黏附能力也得到了验证。此外，L33 包含一个胞外多糖（EPS）生物合成簇，并且它不携带可转移的抗生素抗性基因。京都基因与基因组百科全书（KEGG）途径和碳水化合物活性酶（CAZymes）分析表明，L33 拥有七种氨基酸的生物合成途径，同时它可以降解多种碳水化合物。同时，L33 的直系同源群（COG）和 KEGG 图谱与 26 种 菌株以及两种有充分记录的 益生菌菌株的图谱相似。总之，L33 展现出良好的益生菌潜力，尽管需要进一步研究来阐明其生物学特性的程度。
