Department of Ophthalmology and Visual Sciences, SUNY Upstate Medical University, Syracuse, NY, USA.
Department of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, NY, USA.
Methods Mol Biol. 2025;2858:17-29. doi: 10.1007/978-1-0716-4140-8_2.
The extracellular microenvironment plays a crucial role in regulating a wide range of cell behaviors. Biopolymer hydrogels are ideally suited to present a realistic three-dimensional extracellular milieu to cells in vitro. Here, we describe the fabrication and use of soft tissue-mimetic extracellular matrix protein hydrogels for investigations of human trabecular meshwork cell biology.
细胞外微环境在调节广泛的细胞行为方面起着至关重要的作用。生物聚合物水凝胶非常适合在体外为细胞提供逼真的三维细胞外环境。在这里，我们描述了软组织模拟细胞外基质蛋白水凝胶的制备和使用，用于研究人眼小梁网细胞的生物学。
