Ueda T, Matsumoto K, Akitaya T, Kobatake Y
Exp Cell Res. 1986 Feb;162(2):486-94. doi: 10.1016/0014-4827(86)90352-6.
Spatio-temporal organization of a migrating plasmodium was studied both by analysing intracellular concentrations of adenine and cyclic nucleotides and by applying image processing for recording oscillatory changes in thickness with use of microcomputers. ATP and ADP concentrations were about twice as high in the front as in the rear, while AMP distributed uniformly. On the other hand, cAMP and cGMP concentrations were several times higher in the rear than in the front, showing oscillations in between. The cAMP concentrations at the front oscillated with a phase advancing about one-third of the period with respect to the phase of the thickness oscillation, while cGMP concentration there varied only little. ATP concentration oscillated concomitantly with H+. A feedback control loop consisting of (ATP-H+)-cAMP-Ca2+ is proposed. The possible mechanism of rhythmic contractions involving mitochondria which may excrete pulses of Ca2+ and induce cell polarization is discussed.
通过分析腺嘌呤和环核苷酸的细胞内浓度以及运用图像处理技术借助微型计算机记录厚度的振荡变化，对迁移中的疟原虫的时空组织进行了研究。ATP和ADP的浓度在前部约为后部的两倍，而AMP分布均匀。另一方面，cAMP和cGMP的浓度在后部比前部高几倍，且在两者之间呈现振荡。前部的cAMP浓度振荡的相位相对于厚度振荡的相位提前约三分之一周期，而那里的cGMP浓度变化很小。ATP浓度与H⁺同步振荡。提出了一个由(ATP-H⁺)-cAMP-Ca²⁺组成的反馈控制回路。讨论了涉及线粒体的节律性收缩的可能机制，线粒体可能分泌Ca²⁺脉冲并诱导细胞极化。
