Department of Infection and Immunity, Luxembourg Institute of Health, Strassen, Luxembourg.
Department of Life Sciences and Medicine, University of Luxembourg, Belvaux, Luxembourg.
Expert Rev Proteomics. 2024 Sep-Oct;21(9-10):367-376. doi: 10.1080/14789450.2024.2413092. Epub 2024 Oct 24.
The introduction of trapped ion mobility spectrometry (TIMS) in combination with fast high-resolution time-of-flight (TOF) mass spectrometry to the proteomics field led to a jump in protein identifications and quantifications, as well as a lowering of the limit of detection for proteins from biological samples. Parallel Accumulation-Serial Fragmentation (PASEF) is a driving force for this development and has been adapted to discovery as well as targeted proteomics.
Over the last decade, the PASEF concept has been optimized and led to the implementation of eleven new measurement techniques. In this review, we describe all currently described PASEF measurement techniques and their application to clinical proteomics. Literature was searched using PubMed and Google Scholar search engines.
The use of a dual TIMS tunnel has revolutionized the depth and the speed of proteomics measurements. Currently, we witness how this technique is pushing clinical proteomics forward.
将被困离子淌度谱（TIMS）与快速高分辨率飞行时间（TOF）质谱联用引入蛋白质组学领域，大大提高了蛋白质的鉴定和定量能力，同时降低了生物样品中蛋白质的检测限。平行累积-串联碎裂（PASEF）是推动这一发展的动力，已被应用于发现和靶向蛋白质组学。
在过去的十年中，PASEF 概念得到了优化，并催生了十一种新的测量技术。在这篇综述中，我们描述了所有目前描述的 PASEF 测量技术及其在临床蛋白质组学中的应用。文献检索使用了 PubMed 和 Google Scholar 搜索引擎。
双 TIMS 隧道的使用彻底改变了蛋白质组学测量的深度和速度。目前，我们看到这项技术正在推动临床蛋白质组学的发展。
