Molony L, Hagen P O, Schachat F H
Exp Cell Res. 1986 Mar;163(1):78-86. doi: 10.1016/0014-4827(86)90559-8.
We have examined the intermediate filament (IF) protein content of vascular smooth muscle (SM) cells from several arteries and veins in rabbits and quantitated the changes which occur in SM cell expression of these proteins in response to cholesterol feeding. Cells from control rabbit arteries expressed 30% of their IF protein as desmin, while veins expressed 50% as desmin. During development of diet-induced atherosclerosis, morphological changes in arterial SM cells in the intima correlate with changes in IF expression. There is a significant increase in total IF protein content, vimentin increased differentially in thoracic aorta and desmin in pulmonary artery. In abdominal aorta both increase equally. Cholesterol feeding also resulted in changes in the expression of subspecies of desmin, vimentin, and actin in the thoracic arch. Although cholesterol feeding did not produce obvious morphological changes in the veins examined, venous SM IF protein expression was also altered. In the vena cava of cholesterol-fed rabbits there was an increase in vimentin expression without the parallel increase in desmin that occurred in the arterial system. These studies show that cholesterol feeding of rabbits induces measurable changes in the amounts of IF proteins in both arterial atherosclerotic lesions and venous SM cells.
我们检测了来自兔多条动脉和静脉的血管平滑肌（SM）细胞的中间丝（IF）蛋白含量，并对这些蛋白在SM细胞中的表达因喂食胆固醇而发生的变化进行了定量分析。来自对照兔动脉的细胞将其IF蛋白的30%表达为结蛋白，而静脉细胞则将50%表达为结蛋白。在饮食诱导的动脉粥样硬化发展过程中，内膜中动脉SM细胞的形态变化与IF表达的变化相关。IF蛋白总含量显著增加，波形蛋白在胸主动脉中差异增加，结蛋白在肺动脉中增加。在腹主动脉中两者均同等增加。喂食胆固醇还导致胸主动脉中结蛋白、波形蛋白和肌动蛋白亚型的表达发生变化。虽然喂食胆固醇在检测的静脉中未产生明显的形态变化，但静脉SM的IF蛋白表达也发生了改变。在喂食胆固醇的兔的腔静脉中，波形蛋白表达增加，而结蛋白没有像在动脉系统中那样同时增加。这些研究表明，给兔喂食胆固醇会在动脉粥样硬化病变和静脉SM细胞中诱导IF蛋白量的可测量变化。
