Seed T M, Husseini S G, Knospe W H
Exp Hematol. 1986 Feb;14(2):108-18.
The ultrastructure of developing osseous and hematopoietic tissue and supporting stroma was examined within intraperitoneally implanted, marrow-coated cellulose ester membranes (CEMs). During initial periods of implantation (two weeks), coated CEMs were shallowly infiltrated and surface-lined with mainly two stromal cell types--primitive mesenchymal cells and large, pleomorphic, multinucleated monocytoid-like stromal cells--and, in addition, endothelial cells in early stages of vessel development. Selective proliferation, maturation, and orientation of these cell types along the CEM's surface (one month), resulted in the formation of primitive osseous and hematopoietic tissue sites. Osseous sites developed as undifferentiated mesenchymal cells transformed into well-differentiated secretory cells residing in an electron-dense, pre-mineralized extracellular matrix, that upon extended implantation (3-6 months) formed bone. Hematopoietic sites developed as mesenchymal cells, in intimate contact with the monocytoid-like stromal cells, extended elongated branches into the medullary cavity, and enveloped newly formed surface-associated vascular structures. With subsequent maturation of vascular sinuses and supporting adventitial stroma (3-6 months), the sites were colonized with either uni-, bi-, or trilineal hematopoietic elements. These observations provide evidence that common, marrow-derived precursor stromal cells, i.e., mesenchymal and multinucleated monocytoid-like cells, cooperate in the development of both osseous and hemic tissue sites.
在腹腔内植入的骨髓包被的纤维素酯膜（CEMs）内，对发育中的骨组织、造血组织及支持性基质的超微结构进行了检查。在植入初期（两周），包被的CEMs被浅层浸润，表面主要衬有两种基质细胞类型——原始间充质细胞和大的、多形的、多核单核细胞样基质细胞，此外还有血管发育早期的内皮细胞。这些细胞类型沿CEM表面的选择性增殖、成熟和定向（一个月），导致了原始骨组织和造血组织部位的形成。骨组织部位是随着未分化的间充质细胞转化为位于电子致密的、预矿化细胞外基质中的高度分化的分泌细胞而发育形成的，在延长植入（3 - 6个月）后形成骨。造血组织部位的发育过程是，间充质细胞与单核细胞样基质细胞紧密接触，向髓腔内延伸出细长的分支，并包绕新形成的表面相关血管结构。随着血管窦和支持性外膜基质的后续成熟（3 - 6个月），这些部位被单系、双系或三系造血成分定植。这些观察结果提供了证据，即常见的、源自骨髓的前体基质细胞，即间充质细胞和多核单核细胞样细胞，在骨组织和造血组织部位的发育中协同作用。
