Kelly G M, Druse M J, Tonetti D A, Oden B G
Exp Neurol. 1986 Feb;91(2):219-28. doi: 10.1016/0014-4886(86)90063-4.
We examined the influence of chronic maternal ethanol consumption on the Na+- and Ca2+-independent binding of L-glutamate to synaptic plasma membranes from whole brain as well as from cortices and cerebella of developing offspring. The maximum specific binding (Bmax) of L-glutamate to the Na+- and Ca2+-independent binding sites in synaptic plasma membranes of brain peaked at 17 days of age in the offspring of both control and ethanol-fed rats, although at that age there were significantly fewer binding sites in the brains of the offspring of ethanol-fed rats. The regional localization of this deficit is not now known. However, it appears that one major glutamatergic region (the cortex) does not reflect the transient deficiency of L-glutamate sites in brain. In fact, the concentration of L-glutamate binding sites in cortical synaptic plasma membranes was significantly increased in the 20-day-old offspring of ethanol-fed rats. In contrast to the cortex, binding to cerebellar synaptic plasma membranes was comparable in 20-day-old offspring of control and ethanol-fed rats. Despite transient alterations in the concentrations of L-glutamate binding sites in brain and synaptic plasma membranes, the affinity of the sites for L-glutamate (Kd) was consistently normal in the 14- to 26-day-old offspring of ethanol-fed rats.
我们研究了母体长期摄入乙醇对L-谷氨酸与发育中后代全脑以及皮质和小脑突触质膜上Na⁺和Ca²⁺非依赖性结合的影响。L-谷氨酸与脑突触质膜上Na⁺和Ca²⁺非依赖性结合位点的最大特异性结合（Bmax）在对照大鼠和乙醇喂养大鼠的后代中均在17日龄时达到峰值，尽管在该年龄时，乙醇喂养大鼠后代脑中的结合位点明显较少。目前尚不清楚这种缺陷的区域定位情况。然而，似乎一个主要的谷氨酸能区域（皮质）并未反映出脑中L-谷氨酸位点的短暂缺乏。事实上，在乙醇喂养大鼠20日龄的后代中，皮质突触质膜上L-谷氨酸结合位点的浓度显著增加。与皮质相反，在对照大鼠和乙醇喂养大鼠20日龄的后代中，小脑突触质膜上的结合情况相当。尽管脑和突触质膜中L-谷氨酸结合位点的浓度存在短暂变化，但在乙醇喂养大鼠14至26日龄的后代中，这些位点对L-谷氨酸的亲和力（Kd）始终正常。
