Institute of Biotechnology, Department of Medical Biotechnology and Integrative Physiology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Thandalam, Chennai, 602 105, Tamil Nadu, India.
Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Chinna Kolambakkam, Chengalpattu District, Padalam, India.
Mol Biol Rep. 2024 Oct 22;51(1):1087. doi: 10.1007/s11033-024-09998-1.
An excessive amount of reactive oxygen species triggers oxidative stress, leading to an imbalance in cellular homeostasis. Antioxidant therapy is an effective tool for lowering the oxidative stress and associated ailments. Recently, green nano-based drug formulations have demonstrated promising antioxidant activity and neutralizing oxidative stress. In this study, a tannin molecule Hamamelitannin (HAM), was utilized to synthesize zinc oxide nanoparticles HAM-ZnO NPs, to mitigate oxidative stress and associated ailments .
The HAM-ZnO NPs were synthesized and characterized by XRD, SEM, and FTIR. The antioxidant potentials of HAM-ZnO NPs were analyzed by in vitro antioxidant assays. Zebrafish embryos and larvae were used as in-vivo models to assess the toxicity and antioxidant protective mechanism. Hydrogen peroxide (1mM) was employed to induce oxidative stress and treated with HAM-ZnO NPs to study the cognitive impairment and antioxidant enzyme levels. Levels of reactive oxygen species and cell death due to oxidative stress induction were studied by 2',7'-dichlorodihydrofluorescein diacetate and Acridine orange staining methods. Additionally, expression of Antioxidant genes such as SOD, CAT, GPx, and GSR were studied. .
HAM-ZnO NPs exhibited a spherical morphology and size ranges between 48 and 53 nm. In vitro antioxidant studies revealed the antioxidant properties of HAM-ZnO NPs. Furthermore, in vivo studies indicated that HAM-ZnO NPs don't possess any cytotoxic effects in zebrafish larvae at concentrations between (5-25 µg/ml), The study also observed that HAM-ZnO NPs significantly reduced Hydrogen Peroxide-induced stress and increased antioxidant activity in zebrafish larvae. Also, the antioxidant gene expression was upregulated in the HAM-ZnO NPs zebrafish larvae.
Findings in this study showed that HAM-ZnO NPs might be a potential intervention for diseases linked to oxidative stress.
过量的活性氧会引发氧化应激，导致细胞内稳态失衡。抗氧化治疗是降低氧化应激和相关疾病的有效手段。最近，基于绿色纳米的药物制剂已显示出有希望的抗氧化活性和中和氧化应激的能力。在本研究中，利用单宁分子金缕梅单宁（HAM）合成了氧化锌纳米粒子 HAM-ZnO NPs，以减轻氧化应激和相关疾病。
通过 XRD、SEM 和 FTIR 对 HAM-ZnO NPs 进行了合成和表征。通过体外抗氧化试验分析了 HAM-ZnO NPs 的抗氧化潜力。使用斑马鱼胚胎和幼虫作为体内模型，评估其毒性和抗氧化保护机制。采用 1mM 过氧化氢诱导氧化应激，并使用 HAM-ZnO NPs 处理，研究认知障碍和抗氧化酶水平。通过 2'，7'-二氯二氢荧光素二乙酸酯和吖啶橙染色方法研究了由于氧化应激诱导而产生的活性氧和细胞死亡的水平。此外，还研究了抗氧化基因如 SOD、CAT、GPx 和 GSR 的表达。
HAM-ZnO NPs 呈现出球形形态，尺寸范围在 48 到 53nm 之间。体外抗氧化研究表明 HAM-ZnO NPs 具有抗氧化特性。此外，体内研究表明，在浓度为（5-25μg/ml）的范围内，HAM-ZnO NPs 对斑马鱼幼虫没有任何细胞毒性作用。研究还观察到，HAM-ZnO NPs 可显著减轻过氧化氢诱导的应激，并增加斑马鱼幼虫的抗氧化活性。此外，在 HAM-ZnO NPs 处理的斑马鱼幼虫中，抗氧化基因的表达上调。
本研究结果表明，HAM-ZnO NPs 可能是一种针对与氧化应激相关疾病的潜在干预手段。
