Department of Orthopaedic Surgery, Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College, 152 Ai Guo Road, Nanchang, 330006, Jiangxi, China.
Department of Plastic Surgery, Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College, Jiangxi, China.
Sci Rep. 2024 Oct 22;14(1):24865. doi: 10.1038/s41598-024-76291-3.
To investigate mRNA Expression profile and associated signaling pathways in the treatment of diabetic foot ulcer healing by tibial cortex transverse distraction. Tissue samples were collected from the wound edge before and after the surgery. After reference genome transcriptome sequencing and subsequent bioinformatics analysis, the differentially expressed genes and related pathways were explored, and functional analysis of important genes and pathways was conducted. qRT-PCR was used to verify the significantly expressed genes-HLA-DRB1, HLA-DRB5, CXCL5 and IGFL1. There were 2441 significantly up-regulated and 3904 significantly down-regulated genes in the postoperative group. The qRT-PCR results showed the expression of HLA-DRB1, HLA-DRB5 and CXCL5 was consistent with the transcriptional sequencing results. CXCL5 and CXCL6 differentially up-regulated genes are involved in the process of neovascularization, and HLA-DRB1 is involved in the improvement of the degree of diabetic peripheral nerve degeneration. Pathway analysis showed that differential genes were most significantly enriched in Adherens junction, Inflammatory mediator regulation of TRP channels and Wnt signaling pathway. Inflammatory mediator regulation of TRP channels is involved in the improvement of peripheral neurodegeneration, VEGF signaling pathway is involved in the process of neovascularization, and Wnt signaling pathway is involved in the process of bone healing. Significantly up-regulated CXCL5 and CXCL6 and enriched VEGF signaling pathway analyzed are involved in postoperative lower limb neovascularization. The HLA-DRB1 and the enriched Inflammatory mediator regulation of TRP channels may be related to the improvement of postoperative peripheral neurodegeneration. The differentially expressed genes and related pathways can provide objective basis for further mechanism study.
为了研究胫骨皮质横向牵张术治疗糖尿病足溃疡愈合的 mRNA 表达谱及相关信号通路，我们采集了手术前后创面边缘的组织样本。在参考基因组转录组测序和随后的生物信息学分析后，我们探讨了差异表达基因和相关通路，并对重要基因和通路进行了功能分析。qRT-PCR 用于验证显著表达基因 HLA-DRB1、HLA-DRB5、CXCL5 和 IGFL1。术后组有 2441 个显著上调和 3904 个显著下调基因。qRT-PCR 结果显示 HLA-DRB1、HLA-DRB5 和 CXCL5 的表达与转录组测序结果一致。CXCL5 和 CXCL6 差异上调基因参与了新生血管化过程，而 HLA-DRB1 则参与了改善糖尿病周围神经变性的程度。通路分析表明，差异基因在粘着连接、TRP 通道炎症介质调节和 Wnt 信号通路中最为显著富集。TRP 通道的炎症介质调节参与了周围神经变性的改善，VEGF 信号通路参与了新生血管化过程，Wnt 信号通路参与了骨愈合过程。显著上调的 CXCL5 和 CXCL6 以及富集的 VEGF 信号通路分析参与了术后下肢新生血管化。HLA-DRB1 和富集的 TRP 通道炎症介质调节可能与术后周围神经退行性变的改善有关。差异表达基因和相关通路可为进一步的机制研究提供客观依据。
