Afrin Syeda Rubaiya, Islam Mohammad Rashedul, Alam Md Ashraful, Nisat Ummah Tasnim, Akter Bakul, Hossain Mohammed Kamrul
Department of Pharmacy, Faculty of Biological Sciences, University of Chittagong, Chittagong, Bangladesh.
Department of Pharmacy, Jashore University of Science and Technology, Bangladesh.
BioTechnologia (Pozn). 2024 Sep 30;105(3):249-261. doi: 10.5114/bta.2024.141804. eCollection 2024.
Since plant-based natural drugs are widely accepted in modern times and possess numerous pharmacological effects with an extensive therapeutic range, an ethnomedicinal plant native to Bangladesh was selected to investigate for investigation of its various pharmacological effects. has been traditionally used and has demonstrated numerous pharmacological effects in preclinical investigations. Therefore, this research aimed to assess the central nervous system (CNS) depressant and antioxidant activities of the crude methanol extracts of the stem barks (MDMS), leaves (MDML), and their different fractions.
The CNS depressant activity was assessed using the hole cross, rota-rod, and elevated plus maze tests on Swiss Albino mice, while the antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl free radical, hydrogen peroxide (HO) nonradical scavenging, and ferric reducing power assays.
The conducted assays successfully demonstrated that the chloroform fraction of MDML is a significantly ( < 0.001) effective CNS depressant, muscle relaxant, and anxiolytic agent with excellent antioxidative effects compared to standard and control. The aqueous fraction of MDML also acted as a significantly ( < 0.001) active CNS depressant and muscle relaxant, and it was a highly active ferric-reducing agent. All effects were dose and concentration-dependent.
The presence of various phytochemicals might contribute to these activities. However, further research is suggested to isolate their active compounds and evaluate their mechanisms of action.
由于植物源天然药物在现代被广泛接受，且具有多种药理作用和广泛的治疗范围，因此选择一种原产于孟加拉国的民族药用植物来研究其各种药理作用。该植物在传统上一直被使用，并且在临床前研究中已显示出多种药理作用。因此，本研究旨在评估该植物茎皮粗甲醇提取物（MDMS）、叶粗甲醇提取物（MDML）及其不同馏分的中枢神经系统（CNS）抑制和抗氧化活性。
使用洞板试验、转棒试验和高架十字迷宫试验对瑞士白化小鼠评估中枢神经系统抑制活性，同时使用2,2-二苯基-1-苦基肼自由基、过氧化氢（HO）非自由基清除和铁还原能力测定法评估抗氧化活性。
所进行的试验成功表明，与标准品和对照相比，MDML的氯仿馏分是一种显著（<0.001）有效的中枢神经系统抑制剂、肌肉松弛剂和抗焦虑剂，具有出色的抗氧化作用。MDML的水相馏分也作为一种显著（<0.001）活性的中枢神经系统抑制剂和肌肉松弛剂，并且是一种高活性的铁还原剂。所有作用均呈剂量和浓度依赖性。
各种植物化学物质的存在可能有助于这些活性。然而，建议进一步研究以分离其活性化合物并评估其作用机制。
