Kumar S, Madaan R, Sharma A
S. D. College of Pharmacy, Barnala-148 101, India.
Indian J Pharm Sci. 2008 Nov;70(6):847-51. doi: 10.4103/0250-474X.49143.
An HPTLC densitometric method has been developed to estimate apigenin in Turnera aphrodisiaca aerial and its segregated parts (leaves, stems, flowers and fruits) so that plant can be standardized on the basis of its bioactive marker. The apigenin content in methanol extract of T. aphrodisiaca aerial parts was found to be about fourteen times less than acid hydrolyzed methanol extract of the plant indicating the presence of most of apigenin in glycosidic form. Amongst different plant parts, flowers possessed maximum content of apigenin followed by leaves. The apigenin content was also determined in three marketed formulations of T. aphrodisiaca viz., NLK, DWSG and SBL. DWSG contained higher content of apigenin. Aerial parts of the plant were collected at bimonthly intervals over a period of one year in the months of January, March, May, July, September and November. The plant material collected in September showed maximum content of apigenin.
已开发出一种高效薄层色谱密度测定法来估算特纳草地上部分及其分离部分（叶、茎、花和果实）中的芹菜素，以便能根据其生物活性标志物对该植物进行标准化。发现特纳草地上部分甲醇提取物中的芹菜素含量比该植物的酸水解甲醇提取物中的芹菜素含量少约十四倍，这表明大部分芹菜素以糖苷形式存在。在不同的植物部位中，花中芹菜素含量最高，其次是叶。还测定了三种市售的特纳草制剂（即NLK、DWSG和SBL）中的芹菜素含量。DWSG中芹菜素含量较高。在一年中的1月、3月、5月、7月、9月和11月，每隔两个月采集一次该植物的地上部分。9月采集的植物材料中芹菜素含量最高。
