Besch G J, Tanner M A, Sattler C A, Wolberg W H, Howard S P, Gould M N
Int J Radiat Oncol Biol Phys. 1986 Jan;12(1):75-81. doi: 10.1016/0360-3016(86)90418-9.
A human in vivo-in vitro model for breast cancer has been developed based on culture methods that were systematically optimized for solid breast carcinomas. A V-79 cell clonal agar-based model was used to define the analysis problem encountered with agar based clonogenic survival assays. Based on this, we established methods for the generation and analysis of the survival of primary and recurrent breast carcinomas following irradiation. Four out of five primary breast carcinomas studied had similar radiation-cell survival curves. The fifth tumor was more radioresistant. Interestingly, a recurrent breast carcinoma arising in a heavily irradiated chest wall was no more radioresistant than our series of unirradiated primary carcinomas. These methods may be useful both for the study of the radiobiology of human neoplasms and for customizing the treatments and prognosis of individual patients.
基于对实体乳腺癌进行系统优化的培养方法，已开发出一种用于乳腺癌的人体体内 - 体外模型。使用基于V - 79细胞克隆琼脂的模型来界定基于琼脂的克隆形成存活测定所遇到的分析问题。基于此，我们建立了对原发性和复发性乳腺癌放疗后存活情况进行生成和分析的方法。所研究的五例原发性乳腺癌中有四例具有相似的放射细胞存活曲线。第五个肿瘤对放疗更具抗性。有趣的是，在受重度照射的胸壁上出现的复发性乳腺癌并不比我们一系列未受照射的原发性癌更具放疗抗性。这些方法对于人类肿瘤放射生物学的研究以及针对个体患者定制治疗方案和预测预后可能都很有用。
