Wang Yiping, Wu Wentao, Wang Risheng, Chen Jinwei, Xu Xiangping, Li Meiqi, Jia Chiyu, Chen Nian
Center of Burn & Plastic and Wound Healing Surgery, Hengyang Medical School, The First Affiliated Hospital, University of South China, Hengyang, China.
Department of Burns and Plastic Surgery, The Affiliated Zhuzhou Hospital Xiangya Medical College CSU, Zhuzhou, China.
J Cosmet Dermatol. 2025 Jan;24(1):e16644. doi: 10.1111/jocd.16644. Epub 2024 Oct 23.
To investigate the role of miR-30a-5p on the proliferation and apoptosis of hair follicle stem cells (HFSCs) and whether the Wnt/β-catenin signaling pathway is involved.
HFSCs derived from the vibrissa of mammary rats were obtained by enzymatic digestion, and subsequently the obtained HFSCs were treated with Lipofectamine 2000 cell transfection and divided into normal cell culture group (control), miR-30a-5p overexpression group (miR-30a-5p mimic), miR-30a-5p empty vector group (miR-NC), miR-30a-5p inhibitor group (in-miR-30a-5p), and in-miR-30a-5p empty vector group (in-miR-NC). After transfection, the cell proliferation and apoptosis rates were examined separately. In addition, the mRNA expression of β-catenin, proliferating cell nuclear antigen (PCNA) and apoptosis-related genes (Bax and Bcl-2) were examined.
The results of cell proliferation ability showed that in-miR-30a-5p group promoted cell proliferation of HFSCs relative to other groups, along with significant upregulation of gene levels of PCNA. Apoptosis analysis indicated that apoptosis rate was reduced in the in-miR-30a-5p group, and the expression of Bax was suppressed, while that of Bcl-2 was promoted. Wnt/β-catenin signaling pathway investigation revealed a significant increase in the levels of β-catenin in HFSCs in the in-miR-30a-5p group.
Downregulation of miR-30a-5p levels inhibited HFSCs apoptosis and simultaneously promoted proliferation, furthermore, the increased expression of β-catenin indirectly confirmed the activation of the Wnt/β-catenin signaling pathway.
探讨miR-30a-5p对毛囊干细胞（HFSCs）增殖和凋亡的作用，以及Wnt/β-连环蛋白信号通路是否参与其中。
通过酶消化法获取来自成年大鼠触须的HFSCs，随后将获得的HFSCs用Lipofectamine 2000细胞转染试剂处理，并分为正常细胞培养组（对照组）、miR-30a-5p过表达组（miR-30a-5p模拟物）、miR-30a-5p空载体组（miR-NC）、miR-30a-5p抑制剂组（in-miR-30a-5p）和in-miR-30a-5p空载体组（in-miR-NC）。转染后，分别检测细胞增殖率和凋亡率。此外，检测β-连环蛋白、增殖细胞核抗原（PCNA）和凋亡相关基因（Bax和Bcl-2）的mRNA表达。
细胞增殖能力结果显示，相对于其他组，in-miR-30a-5p组促进了HFSCs的细胞增殖，同时PCNA基因水平显著上调。凋亡分析表明，in-miR-30a-5p组的凋亡率降低，Bax表达受到抑制，而Bcl-2表达得到促进。Wnt/β-连环蛋白信号通路研究显示，in-miR-30a-5p组HFSCs中β-连环蛋白水平显著升高。
miR-30a-5p水平下调抑制了HFSCs凋亡，同时促进了增殖，此外，β-连环蛋白表达增加间接证实了Wnt/β-连环蛋白信号通路的激活。
