一种诊断霍乱弧菌的简易实验室方法。

A simple laboratory method for the diagnosis of V. cholerae.

作者信息

Huq M I

出版信息

Trans R Soc Trop Med Hyg. 1979;73(5):553-6. doi: 10.1016/0035-9203(79)90050-6.

Abstract

In many developing countries where cholera occurs, bacteriological facilities at the local level are lacking and either cholera may be unreported or other diarrhoeal diseases may be mistakenly reported as cholera. A simple and practical method, suitable for use in small hospitals and health centres and by field teams of health workers is described. Swab samples are streaked on to various media (TTGA and TCBS were found to be the most satisfactory), incubated overnight at 35 degrees C and read. The characteristics which distinguish Vibrio cholerae colonies from others developing are described. In tests on 309 cholera patients, culture on TTGA gave 100% and on TCBS 96.7% positives.

摘要

在许多有霍乱发生的发展中国家，地方层面缺乏细菌学检测设施，霍乱可能未被报告，或者其他腹泻疾病可能被误报为霍乱。本文描述了一种简单实用的方法，适用于小型医院、健康中心以及卫生工作者现场团队。将拭子样本接种在各种培养基上（发现TTGA和TCBS是最理想的），于35摄氏度培养过夜后进行观察。文中描述了霍乱弧菌菌落与其他生长菌落相区别的特征。在对309例霍乱患者的检测中，TTGA培养基培养的阳性率为100%，TCBS培养基培养的阳性率为96.7%。