Department of Cardiology, First Affiliated Hospital of Ningbo University (Ningbo First Hospital), School of Medicine, Ningbo University, Ningbo 315211, China.
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
Anal Chem. 2024 Nov 5;96(44):17800-17806. doi: 10.1021/acs.analchem.4c04159. Epub 2024 Oct 23.
Cardiovascular disease, a chronic and progressive arterial wall disease, is increasingly recognized for its clinical significance. Aminopeptidases N (APN), crucial in the pathophysiological processes of vulnerable plaque, have been linked to endothelial dysfunction, oxidative stress, and plaque formation, thus highlighting their potential as biomarkers for disease progression. However, current detection methods for APN in body fluids and in vivo have limitations, including insufficient sensitivity and specificity, time delays, and the inability to directly reflect enzyme activity in plaques. To address these challenges, we developed an optical probe, , for in vivo imaging of aminopeptidases, providing a potential implementation in cardiovascular disease. Our work demonstrated the applicability of for specific monitoring of aminopeptidase levels in plaques and serum, shedding light on its potential for further research in cardiovascular disease.
心血管疾病是一种慢性进行性动脉壁疾病，其临床意义日益受到重视。氨基肽酶 N（APN）在易损斑块的病理生理过程中起着关键作用，与内皮功能障碍、氧化应激和斑块形成有关，因此凸显了其作为疾病进展生物标志物的潜力。然而，目前用于检测体液和体内 APN 的方法存在局限性，包括灵敏度和特异性不足、时间延迟以及无法直接反映斑块中的酶活性。为了解决这些挑战，我们开发了一种光学探针 ，用于易损斑块的体内成像，为心血管疾病的应用提供了一种潜在的实现途径。我们的工作证明了 用于特异性监测斑块和血清中氨基肽酶水平的适用性，为其在心血管疾病中的进一步研究提供了思路。
