Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Anal Chem. 2017 Mar 7;89(5):3217-3221. doi: 10.1021/acs.analchem.7b00021. Epub 2017 Feb 23.
An ultrasensitive ratiometric fluorescent probe (CVN) has been designed and synthesized by incorporating alanine into the cresyl violet fluorophore. The probe shows ratiometric fluorescence response toward aminopeptidase N (APN) through the increase of fluorescent intensity ratio of 626/575 nm. The sensitivity of the probe is ultrahigh with a detection limit of 33 pg/mL, which can quantify the contents of APN in 500-fold diluted human urine samples. Furthermore, by using ratiometric fluorescence imaging, the probe reveals significantly higher contents of APN in HepG2 cells than those in LO2 cells, which has been further used to distinguish these two types of cells in mixed cocultures. The probe could be of great importance for the APN-related disease diagnosis and pathophysiology elucidation.
一种超灵敏的比率荧光探针 (CVN) 已经被设计和合成，方法是将丙氨酸整合到 cresyl 紫荧光团中。该探针通过荧光强度比 626/575nm 的增加，对氨肽酶 N (APN) 表现出比率荧光响应。该探针的灵敏度非常高，检测限低至 33pg/mL，可定量分析 500 倍稀释的人尿液样本中的 APN 含量。此外，通过比率荧光成像，该探针显示 HepG2 细胞中的 APN 含量明显高于 LO2 细胞，这已进一步用于区分这两种混合共培养细胞。该探针对于与 APN 相关的疾病诊断和病理生理学阐明具有重要意义。
