Silva Bibiana, Biluca Fabíola Carina, Lubschinski Tainá Larissa, Mohr Eduarda Talita Bramorski, Gonzaga Luciano Valdemiro, Fett Roseane, Dalmarco Eduardo Monguilhott, Costa Ana Carolina Oliveira
Department of Food Science and Technology, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
Department of Clinical Analysis, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
Cell Biochem Biophys. 2025 Jun;83(2):1809-1817. doi: 10.1007/s12013-024-01590-5. Epub 2024 Oct 23.
Macrophages play an important role during the inflammatory process. These cells can adopt either the pro- or anti-inflammatory phenotypes. While stingless bee honeys have demonstrated evidence of anti-inflammatory potential, their capacity to induce a shift from a pro-inflammatory state to an inflammation-resolution state has not been thoroughly investigated. In this study, the anti-inflammatory activity of two stingless bees (Scaptotrigona bicunctata-honey A and Melipona quadriasciata-honey G) honeys in J774 macrophages induced by LPS was evaluated. Both honeys exhibited non-cytotoxic effects and reduced nitrite and IL-4 levels. However, only honey G increased the levels of the anti-inflammatory cytokine IL-13, by 163.1 ± 14.8% (p < 0.05) and was further investigated for its immunomodulatory effect. This honey reduced the expression of TLR4 by 59.3 ± 3.5% (p < 0.001) and increased the mannose receptor levels by 67.3 ± 2.4% (p < 0.001). Moreover, it increased the phagocytic activity by 25.0 ± 7.7% (p < 0.01) and decreased the death of the macrophages by 32.1 ± 1.7% (p < 0.001). Collectively, these findings highlight stingless bee honey from Melipona quadriasciata bee has an important immunomodulatory effect, as it reduces the markers of the pro-inflammatory state of J774 cells and increases the markers of resolution or anti-inflammatory responses.
巨噬细胞在炎症过程中发挥着重要作用。这些细胞可呈现促炎或抗炎表型。虽然无刺蜂蜂蜜已显示出具有抗炎潜力的证据，但其诱导从促炎状态转变为炎症消退状态的能力尚未得到充分研究。在本研究中，评估了两种无刺蜂（Scaptotrigona bicunctata - 蜂蜜A和Melipona quadriasciata - 蜂蜜G）蜂蜜对LPS诱导的J774巨噬细胞的抗炎活性。两种蜂蜜均表现出无细胞毒性作用，并降低了亚硝酸盐和IL - 4水平。然而，只有蜂蜜G使抗炎细胞因子IL - 13水平升高了163.1±14.8%（p < 0.05），并对其免疫调节作用进行了进一步研究。这种蜂蜜使TLR4的表达降低了59.3±3.5%（p < 0.001），并使甘露糖受体水平升高了67.3±2.4%（p < 0.001）。此外，它使吞噬活性提高了25.0±7.7%（p < 0.01），并使巨噬细胞死亡减少了32.1±1.7%（p < 0.001）。总的来说，这些发现突出表明，来自Melipona quadriasciata蜂的无刺蜂蜂蜜具有重要的免疫调节作用，因为它降低了J774细胞促炎状态的标志物，并增加了炎症消退或抗炎反应的标志物。
