Wang Miao, Sun He-Nan, Liu Xing-Yu, Liu Mingjun, Li Shan-Shan
Institute for Chemical Biology & Biosensing, College of Life Sciences, Qingdao University, 308 Ningxia Road, Qingdao 266071, China.
Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao 266003, China.
Bioelectrochemistry. 2025 Feb;161:108838. doi: 10.1016/j.bioelechem.2024.108838. Epub 2024 Oct 16.
Cardiac troponin I (cTnI) has been widely used in clinical diagnosis of acute myocardial infarction (AMI). Herein, a sensitive electrochemical biosensor for cTnI analysis was designed, in which the simple synthesized Pd@PdPtCo mesoporous nanopolyhedras (MNPs) were utilized as signal amplifiers. The mesoporous polyhedral structure of Pd@PdPtCo MNPs endows them with more specific surface area and more active sites, as well as the synergistic effect between multiple metal elements, all of which increase the electrocatalytic performance of Pd@PdPtCo MNPs in efficiently oxidizing hydroquinone (HQ) to benzoquinone (BQ). Experimental results showed that Pd@PdPtCo MNPs had better performance in oxidation of HQ to BQ compared with their corresponding monometallic and bimetallic nanomaterials. With the aid of the interaction between antigens and antibodies, the peak current of HQ to BQ showed an upward trend with increasing concentration of cTnI, thus the quantitative detection of cTnI could be achieved. Under optimal conditions, the biosensor prepared in this work has a wider linear range (1.0 × 10-200 ng mL) and a lower detection limit (0.031 pg mL) than other sensors reported in literatures, coupled by good stability and high sensitivity. More importantly, it also performed well in complex serum environment, proving that the electrochemical sensor has a practical application potential in this field.
心肌肌钙蛋白I（cTnI）已广泛应用于急性心肌梗死（AMI）的临床诊断。在此，设计了一种用于cTnI分析的灵敏电化学生物传感器，其中简单合成的钯@钯铂钴介孔纳米多面体（MNPs）被用作信号放大器。钯@钯铂钴MNPs的介孔多面体结构赋予它们更大的比表面积和更多的活性位点，以及多种金属元素之间的协同效应，所有这些都提高了钯@钯铂钴MNPs在将对苯二酚（HQ）有效氧化为苯醌（BQ）方面的电催化性能。实验结果表明，与相应的单金属和双金属纳米材料相比，钯@钯铂钴MNPs在将HQ氧化为BQ方面具有更好的性能。借助抗原与抗体之间的相互作用，HQ到BQ的峰值电流随着cTnI浓度的增加呈上升趋势，从而可以实现cTnI的定量检测。在最佳条件下，本工作制备的生物传感器具有比文献报道的其他传感器更宽的线性范围（1.0×10⁻²⁰⁰ ng/mL）和更低的检测限（0.031 pg/mL），同时具有良好的稳定性和高灵敏度。更重要的是，它在复杂的血清环境中也表现良好，证明该电化学生物传感器在该领域具有实际应用潜力。
