Aiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California, San Diego, La Jolla, CA, USA.
Shu and K.C. Chien and Peter Farrell Collaboratory, University of California, San Diego, La Jolla, CA, USA.
Commun Biol. 2024 Oct 24;7(1):1382. doi: 10.1038/s42003-024-06982-0.
Cowpea mosaic virus (CPMV) is a plant virus that is currently being developed for intratumoral immunotherapy. CPMV relieves the immune system from tumor-induced immunosuppression; reprograms the tumor microenvironment to an activated state whereby the treated and distant tumors are recognized and eradicated. Toward translational studies, we investigated the safety of CPMV, specifically addressing whether pathogenicity would be induced in mammalian cells. We show that murine macrophage immune cells recognize CPMV; however, there is no indication of de novo viral protein synthesis or RNA replication. Furthermore, we show that CPMV does not induce hemolysis, platelet aggregation and plasma coagulation amongst other assays in human blood and immune cells. Taken together, we anticipate that these results will reinforce the development of CPMV as an immunotherapeutic platform.
豇豆花叶病毒（CPMV）是一种植物病毒，目前正被开发用于肿瘤内免疫疗法。CPMV 缓解免疫系统因肿瘤引起的免疫抑制；将肿瘤微环境重新编程为激活状态，使治疗和远处的肿瘤被识别并消除。在转化研究方面，我们研究了 CPMV 的安全性，特别关注是否会在哺乳动物细胞中诱导致病性。我们表明，鼠巨噬细胞免疫细胞识别 CPMV；然而，没有新的病毒蛋白合成或 RNA 复制的迹象。此外，我们表明 CPMV 不会在人血和免疫细胞中引起溶血、血小板聚集和血浆凝固等其他检测。总之，我们预计这些结果将加强 CPMV 作为免疫治疗平台的开发。
