Braithwaite A W
J Gen Virol. 1986 Feb;67 ( Pt 2):391-6. doi: 10.1099/0022-1317-67-2-391.
Human adenovirus type 5 is shown to cause a limited infection of the cells lining the ventricles of 2- to 3-week-old newborn rats. By the distribution of fluorescent antibody-labelled cells the infection appears to be confined to the ependymal cells. In these same (ependymal) cells autoradiographic labelling of [3H]thymidine-pulsed brains indicates that viral and possibly cellular DNA synthesis is occurring. Studies with the mutants dl 312 and dl 313 indicate that some cellular DNA synthesis is indeed occurring. No DNA synthesis is observed in cells after inoculation of brains with phosphate-buffered saline or infection of brains with Semliki Forest virus. These results suggest that expression of adenovirus early region genes stimulates differentiated (G0) brain cells to enter a cell cycle.
已证实，人5型腺病毒会导致2至3周龄新生大鼠脑室衬里细胞发生有限感染。通过荧光抗体标记细胞的分布情况，感染似乎局限于室管膜细胞。在这些相同的（室管膜）细胞中，对[3H]胸苷脉冲处理的大脑进行放射自显影标记表明，病毒以及可能的细胞DNA合成正在发生。对突变体dl 312和dl 313的研究表明，确实正在发生一些细胞DNA合成。在用磷酸盐缓冲盐水接种大脑或用Semliki森林病毒感染大脑后，未在细胞中观察到DNA合成。这些结果表明，腺病毒早期区域基因的表达会刺激分化的（G0期）脑细胞进入细胞周期。
